59767

Chlorphenolrot-β-D-galactopyranosid

β-galactosidase, ≥90% (HPLC), powder or crystals

• Synonym(e): CPRG, Chlorphenol Rot β-D-galactosid
• Empirische Formel (Hill-System): C₂₅H₂₂Cl₂O₁₀S
• CAS-Nummer: 99792-79-7
• Molekulargewicht: 585.41
• UNSPSC Code: 12352204
• PubChem Substance ID: 57651696
• NACRES: NA.83
• MDL number: MFCD05863726

Eigenschaften

• Produktname: Chlorphenolrot-β-D-galactopyranosid, ≥90% (HPLC)
• assay: ≥90% (HPLC)
• form: powder or crystals
• solubility: water: 20 mg/mL, clear, orange to very deep red
• storage temp.: −20°C
• SMILES string: OC[C@H]1O[C@@H](Oc2ccc(cc2Cl)C(=C3/C=CC(=O)C(Cl)=C3)\c4ccccc4S(O)(=O)=O)[C@H](O)[C@@H](O)[C@H]1O
• InChI: 1S/C25H22Cl2O10S/c26-15-9-12(5-7-17(15)29)21(14-3-1-2-4-20(14)38(33,34)35)13-6-8-18(16(27)10-13)36-25-24(32)23(31)22(30)19(11-28)37-25/h1-10,19,22-25,28,30-32H,11H2,(H,33,34,35)/b21-12-/t19-,22+,23+,24-,25-/m1/s1
• InChI key: YOUWVNCQJOMMEU-AETRGLENSA-N

Beschreibung

General description

Chlorophenol red-β-D-galactopyranoside (CPRG) is a long-wavelength yellow dye commonly used for colorimetric assays. It serves as a substrate for β-galactosidase (β-gal) which hydrolyzes CPRG to form chlorophenol red (purple colored). This product is quantified by measuring its absorbance at 570 nm. CPRG is used to identify fecal coliform bacteria. It is about 10 times more sensitive than other chromogenic agents such as o-nitrophenyl-β-galactoside (ONPG). CPRG also has a high rapid turnover.

Application

Chlorophenol Red-β-D-galactopyranoside has been used as a substrate for β-galactosidase (β-gal):

• in paper-based cell-free protein expression system for target RNA detection
• in colorimetric detection of multiple clinically relevant mutations using a lacZ output gene
• in colorimetric assay for bacteria quantification

Other Notes

Measures the expression levels of β-galactosidase in transfected cells. The lacZ gene is a popular reporter gene in transfection reactions because its product, β-galactosidase, is a very stable enzyme.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• Was ist los?: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• PSA (Persönliche Schutzausrüstung): Eyeshields, Gloves, type N95 (US)