88417

Thiazolylblau-formazan

MTT reduction end product, powder

• Synonym(e): 1-(4,5-Dimethylthiazol-2-yl)-3,5-diphenylformazan, MTT-Formazan
• Empirische Formel (Hill-System): C₁₈H₁₇N₅S
• CAS-Nummer: 57360-69-7
• Molekulargewicht: 335.43
• UNSPSC Code: 12352204
• PubChem Substance ID: 329769650
• NACRES: NA.83
• MDL number: MFCD00080731

Eigenschaften

• Produktname: Thiazolylblau-formazan, MTT reduction end product
• Quality Segment: 100
• form: powder
• solubility: chloroform: 10 mg/mL, clear, dark violet
• SMILES string: Cc1nc(\N=N\C(=N\Nc2ccccc2)c3ccccc3)sc1C
• InChI: 1S/C18H17N5S/c1-13-14(2)24-18(19-13)23-22-17(15-9-5-3-6-10-15)21-20-16-11-7-4-8-12-16/h3-12,20H,1-2H3/b21-17+,23-22+
• InChI key: GIFKVQOPAJDJLO-SZVLODPRSA-N

Beschreibung

Application

MTT Formazan has been used in cell viability assay.

Biochem/physiol Actions

MTT assay is a colorimetric reaction, generally used to examine cell viability. It involves the enzymatic reduction of a yellow-colored, water-soluble, 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) to purple-colored, water-insoluble, MTT-formazan. This reaction is catalyzed by mitochondrial dehydrogenase and only occurs in viable cells. Thus, the amount of blue formazan is proportional to the number of viable cells. The absorbance of the formazan solution is analyzed spectrophotometrically at 570 nm.

Other Notes

Reviews: Tetrazolium salts and formazans

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• PSA (Persönliche Schutzausrüstung): Eyeshields, Gloves, type N95 (US)