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88417

Thiazolylblau-formazan

MTT reduction end product, powder

Synonym(e):

1-(4,5-Dimethylthiazol-2-yl)-3,5-diphenylformazan, MTT-Formazan

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Größe/SKUVerfügbarkeitPreis
250 mg
Warenkorb auf Verfügbarkeit prüfen
€ 52,20
1 g
Warenkorb auf Verfügbarkeit prüfen
€ 176,00

Über diesen Artikel

Empirische Formel (Hill-System):
C18H17N5S
CAS-Nummer:
Molekulargewicht:
335.43
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.83
MDL number:

€ 52,20


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Produktname

Thiazolylblau-formazan, MTT reduction end product

Quality Segment

form

powder

solubility

chloroform: 10 mg/mL, clear, dark violet

SMILES string

Cc1nc(\N=N\C(=N\Nc2ccccc2)c3ccccc3)sc1C

InChI

1S/C18H17N5S/c1-13-14(2)24-18(19-13)23-22-17(15-9-5-3-6-10-15)21-20-16-11-7-4-8-12-16/h3-12,20H,1-2H3/b21-17+,23-22+

InChI key

GIFKVQOPAJDJLO-SZVLODPRSA-N

Application

MTT Formazan has been used in cell viability assay.

Biochem/physiol Actions

MTT assay is a colorimetric reaction, generally used to examine cell viability. It involves the enzymatic reduction of a yellow-colored, water-soluble, 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) to purple-colored, water-insoluble, MTT-formazan. This reaction is catalyzed by mitochondrial dehydrogenase and only occurs in viable cells. Thus, the amount of blue formazan is proportional to the number of viable cells. The absorbance of the formazan solution is analyzed spectrophotometrically at 570 nm.

Other Notes

Reviews: Tetrazolium salts and formazans

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Dieser Artikel
M200393145CT02
description

MTT reduction end product

description

powder

description

≥90% (UV)

description

MTT Cell Growth Assay is a colorimetric assay that can be used for either proliferation or complement-mediated cytotoxicity assays.

solubility

chloroform: 10 mg/mL, clear, dark violet

solubility

acetone: 1 mg/mL

solubility

dioxane: 50 mg/mL, clear

solubility

-

Quality Level

100

Quality Level

200

Quality Level

100

Quality Level

100

form

powder

form

powder

form

powder

form

-


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Lagerklasse

11 - Combustible Solids

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

PSA (Persönliche Schutzausrüstung)

Eyeshields, Gloves, type N95 (US)



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Questions

Reviews

Active Filters

    • Review 1
    • Votes 14
    4 out of 5 stars.

    Will Not Work For MTT Proliferation Assay

    I accidentally bought this thinking it was MTT salt to use in a cell proliferation assay but it is MTT Formazan, the water insoluble end product! Therefore, it is useless for the assay. I suppose you could use it as a control to optimize your buffer for solubilizing the insoluble Formazan. The description for this product is misleading and should clarify that this is not what you need for an MTT assay, and refer the customer to MTT salt. That said, this is a nice purple crystalline powder and insoluble in water consistent with MTT Formazan, if that is what you actually want.

    Helpful?