I accidentally bought this thinking it was MTT salt to use in a cell proliferation assay but it is MTT Formazan, the water insoluble end product! Therefore, it is useless for the assay. I suppose you could use it as a control to optimize your buffer for solubilizing the insoluble Formazan. The description for this product is misleading and should clarify that this is not what you need for an MTT assay, and refer the customer to MTT salt. That said, this is a nice purple crystalline powder and insoluble in water consistent with MTT Formazan, if that is what you actually want.
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88417
Thiazolylblau-formazan
MTT reduction end product, powder
Synonym(e):
1-(4,5-Dimethylthiazol-2-yl)-3,5-diphenylformazan, MTT-Formazan
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| Größe/SKU | Verfügbarkeit | Preis |
|---|---|---|
250 mg | Warenkorb auf Verfügbarkeit prüfen | € 52,20 |
1 g | Warenkorb auf Verfügbarkeit prüfen | € 176,00 |
Über diesen Artikel
Empirische Formel (Hill-System):
C18H17N5S
CAS-Nummer:
Molekulargewicht:
335.43
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.83
MDL number:
€ 52,20
Warenkorb auf Verfügbarkeit prüfen
Technischer Dienst
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Unterstützung erhaltenProduktname
Thiazolylblau-formazan, MTT reduction end product
Quality Segment
form
powder
solubility
chloroform: 10 mg/mL, clear, dark violet
SMILES string
Cc1nc(\N=N\C(=N\Nc2ccccc2)c3ccccc3)sc1C
InChI
1S/C18H17N5S/c1-13-14(2)24-18(19-13)23-22-17(15-9-5-3-6-10-15)21-20-16-11-7-4-8-12-16/h3-12,20H,1-2H3/b21-17+,23-22+
InChI key
GIFKVQOPAJDJLO-SZVLODPRSA-N
Biochem/physiol Actions
MTT assay is a colorimetric reaction, generally used to examine cell viability. It involves the enzymatic reduction of a yellow-colored, water-soluble, 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) to purple-colored, water-insoluble, MTT-formazan. This reaction is catalyzed by mitochondrial dehydrogenase and only occurs in viable cells. Thus, the amount of blue formazan is proportional to the number of viable cells. The absorbance of the formazan solution is analyzed spectrophotometrically at 570 nm.
Other Notes
Reviews: Tetrazolium salts and formazans
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Dieser Artikel | |||
|---|---|---|---|
| description MTT reduction end product | description powder | description - | description - |
| solubility chloroform: 10 mg/mL, clear, dark violet | solubility acetone: 1 mg/mL | solubility - | solubility - |
| Quality Level 100 | Quality Level 200 | Quality Level - | Quality Level - |
| form powder | form powder | form - | form - |
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Lagerklasse
11 - Combustible Solids
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
PSA (Persönliche Schutzausrüstung)
Eyeshields, Gloves, type N95 (US)
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