97875

Atto 647

BioReagent, suitable for fluorescence, ≥90% (HPCE)

• UNSPSC Code: 12352108
• NACRES: NA.32
• MDL number: MFCD05865408

Eigenschaften

• product line: BioReagent
• Quality Segment: 100
• assay: ≥90% (HPCE)
• form: powder
• manufacturer/tradename: ATTO-TEC GmbH
• transmittance: 254 nm, 647 nm
• fluorescence: λ_ex 643 nm; λ_em 665 nm in 0.1 M phosphate pH 7.0
• λ: in ethanol (with 0.1% trifluoroacetic acid)
• UV absorption: λ: 645-651 nm A_max
• suitability: suitable for fluorescence
• storage temp.: −20°C

Beschreibung

General description

Atto 647 is a zwitterionic dye with a net electrical charge of zero. It is a pH sensitive product. While practically stable up to pH 8.5, it slowly degrades at higher pH. Atto 647 is a new label with high molecular absorption (120,000) and quantum yield (0.20) as well as enough Stokes shift between excitation and emission maximum. Atto 647 is characterized by a high photostability and brightness. In addition, this label has good water solubility and low triplet formation ability.

Application

Die Atto Fluoreszenzfarbstoffe wurden für die hochsensitive Anwendung bis hin zur Einzel-Molekül-Detektion (SMD) entwickelt. Sie haben eine starre Molekülstruktur, was das Auftreten einer cis-trans-Isomerisierung unterbindet. Daher besitzen diese Farbstoffe eine ausserordentlich hohe Fluoreszenzintensität mit einer geringen spektralen Verschiebung nach der Kopplung an Biomoleküle.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)