W0138

Western Blocker^™ Lösung

for HRP detection systems

• Synonym(e): Western blot blocking solution, blocking buffer
• NACRES: NA.25
• UNSPSC Code: 41105329
• eCl@ss: 32110600

Eigenschaften

• Quality Segment: 200
• shelf life: ≥2 yr
• technique(s): immunodetection: suitable
• storage temp.: 2-8°C

Beschreibung

General description

Western Blocker Solution is used to block both PVDF and nitrocellulose membranes after proteins have been transferred onto them through dot blotting or Western blotting; blocking is performed to reduce or eliminate nonspecific binding of primary or secondary antibodies.

Application

Western Blocker-Lösung wurde für Detektionssysteme mit Meerrettichperoxidase (HRP)-Konjugaten optimiert. Es ist auch mit alkalischer Phosphatase-basierten Systemen kompatibel.

Features and Benefits

• Does not inhibit weak HRP signals, as many milk-based blockers do.
• Can be used both as a blocking component and as a probing component.
• Supplied ready-to-use; no preparative step is required.

Legal Information

Western Blocker is a trademark of Sigma-Aldrich Co. LLC

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• WGK: WGK 2
• Signalwort: Danger
• Gefahrencodes: EUH430
• Sicherheitshinweise: P201 - P202 - P273 - P391 - P405 - P501
• Hazard Classifications: ED ENV 1