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A0810

Endoglycosidase H from Streptomyces plicatus

recombinant, expressed in E. coli, buffered aqueous solution

Synonym(e):

β-N-Acetylglucosaminidase H

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Größe/SKUVerfügbarkeitPreis
1 unit
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€ 574,00

Über diesen Artikel

CAS-Nummer:
UNSPSC Code:
12352204
NACRES:
NA.32
EG-Nummer:
MDL number:
Recombinant:
expressed in E. coli

€ 574,00


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recombinant

expressed in E. coli

Quality Segment

conjugate

(N-linked)

form

buffered aqueous solution

shipped in

wet ice

storage temp.

2-8°C

General description

Endoglycosidase H or endo-β-N-acetylglucosaminidase H is an glycohydrolase. It is produced by Streptomyces plicatus and other Streptomyces species.

Biochem/physiol Actions

Endoglycosidase H is involved in cleaving the N-linked glycans present between the two N-acetylglucosamine (GlcNAc) residues in the core of the glycan chain in high-mannose sugars.

Physical form

Solution in 20 mM Tris HCl, pH 7.5, containing 50 mM NaCl, 1 mM EDTA

Other Notes

One unit will release N-linked oligosaccharides from 1 μmole of denatured ribonuclease B per min at 37 °C at pH 5.5.

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Dieser Artikel
E9762324725324727
description

recombinant, expressed in E. coli, buffered aqueous solution

description

recombinant, expressed in E. coli, ≥16 U/mg, buffered aqueous solution

description

Endoglycosidase F1, Elizabethkingia meningosepticum, Recombinant, E. coli cleaves asparagine-linked or free oligomannose and hybrid. Suitable for deglycosylation of native proteins.

description

Endoglycosidase F3, Elizabethkingia meningosepticum, Recombinant, E. coli, cleaves asparagine-linked or free biantennary and triantennary complex, and Man3GlcNAc oligosaccharides from glycoproteins.

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

form

buffered aqueous solution

form

buffered aqueous solution

form

liquid

form

liquid

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

100

conjugate

(N-linked)

conjugate

(N-linked)

conjugate

(N-linked)

conjugate

(N-linked)


Lagerklasse

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)



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Questions

  1. Could you provide guidance on adjusting the pH of a solution that has a pH of 7.5, even though the workable pH range mentioned in the data sheet is 5.0–6.0? Specifically, which acid would be best to use for adjusting the pH before using the solution?

    1 answer
    1. Please consider that the condition of the stock enzyme is different from the reaction in which it is used. You will be using a small amount of enzyme in a buffered reaction that is adjusted to the optimal pH by the reaction buffer itself.

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