A0847

Adenylyl Cyclase Toxin from Bordetella pertussis

≥70% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, ≥50 units/mg protein

• UNSPSC Code: 41106305
• MDL number: MFCD00675046

Eigenschaften

• recombinant: expressed in E. coli
• Quality Segment: 200
• assay: ≥70% (SDS-PAGE)
• form: lyophilized powder
• specific activity: ≥50 units/mg protein
• mol wt: 177 kDa by amino acid sequence
• storage temp.: 2-8°C
• Gene Information: human ... CAMP(820) ; mouse ... CAMP(12796) ; rat ... CAMP(316010)

Beschreibung

Biochem/physiol Actions

Adenylate cyclase toxin from Bordetella pertussis belongs to the family of pore-forming toxins. It converts host ATP to cyclic AMP (cAMP). May be used to affect intracellular cAMP levels in mammalian cells.

Preparation Note

When reconstituted with 50 μL of water, the protein is in 0.05 M Tris, 8 M urea, 0.002 M EDTA, pH 8.0.

Other Notes

One unit will produce 1.0 μmol cAMP from ATP per minute at pH 8.0 at 30 °C

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• Was ist los?: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• PSA (Persönliche Schutzausrüstung): Eyeshields, Gloves, type N95 (US)