A5384

Aconitase from porcine heart

• Synonym(e): Aconitate hydratase, Citrate (isocitrate) hydrolyase
• CAS-Nummer: 9024-25-3
• EG-Nummer: 4.2.1.3 (BRENDA, IUBMB)
• UNSPSC Code: 12352204
• NACRES: NA.54
• MDL number: MFCD00130335
• Biological source: Porcine heart

Eigenschaften

• biological source: Porcine heart
• Quality Segment: 200
• form: solid
• mol wt: ~66 kDa by gel filtration
• storage temp.: −20°C

Beschreibung

Application

Aconitase may be usefull in diabetes research as well as to study structural conservation between iron-responsive element binding proteins (IRE-BP) and isomerases .

Biochem/physiol Actions

Aconitase catalyses the stereo-specific isomerization of citrate to isocitrate via cis-aconitate in the tricarboxylic acid cycle. Aconitase is inhibited by cyanide, sulfide and copper and mercury at low concentrations. It is competitively inhibited by trans-aconitate. Aconitase contains an internal Fe-S cluster which is responsible for its activity.

Analysis Note

Crude preparation containing aconitase activity

Other Notes

One unit will convert 1.0 μmole of citrate (via cis-aconitate) to isocitrate per min at pH 7.4 at 25 °C.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• Was ist los?: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• PSA (Persönliche Schutzausrüstung): Eyeshields, Gloves, type N95 (US)