A6007

Apotryptophanase aus E. coli

soluble powder, 75-150 units/mg solid

• Synonym(e): Tryptophanase aus E. coli
• CAS-Nummer: 9024-00-4
• UNSPSC Code: 12352204
• NACRES: NA.54
• EG-Nummer: 4.1.99.1 (BRENDA, IUBMB)
• MDL number: MFCD00130539
• Specific activity: 75-150 units/mg solid
• Biological source: Escherichia coli

Eigenschaften

• biological source: Escherichia coli
• Quality Segment: 200
• form: soluble powder
• specific activity: 75-150 units/mg solid
• storage temp.: −20°C

Beschreibung

Application

Apotryptophanase is used for the quantitative determination of pyridoxal-phosphate. Apotryptophanase, from Sigma, has been used to study pregnancy-associated PLP deficiency and vitamin B-6 deficiency .

Biochem/physiol Actions

Apotryptophanase hydrolizes tryptophan and is capable of catalyzing α,β-elimination reactions with a number of substituted amino acids, including S-methyl-, S-ethyl- and S-benzyl- L-cysteine. DTNB inactivates tryptophanase .

Other Notes

One unit releases one μg of indole from L-tryptophan in 10 min at pH 8.3 at 37 °C in the presence of 0.04 mM pyridoxal-5′--phosphate.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• PSA (Persönliche Schutzausrüstung): Eyeshields, Gloves, type N95 (US)