The purification method is proprietary, and testing for other activities, such as alcohol dehydrogenase, is not conducted. The following reference from the Product Information Sheet may be helpful: Bostian, K. A., and Betts, G. F., Rapid purification and properties of potassium-activated aldehyde dehydrogenase from Saccharomyces cerevisiae. Biochemical Journal, 173(3), 773-786 (1978).
Fortfahren mit
A6338
Aldehyd-Dehydrogenase, Kalium-aktiviert aus Backhefe (S. cerevisiae)
lyophilized powder, ≥2.0 units/mg protein
Synonym(e):
Aldehyd:NAD[P]+-Oxidoreduktase
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| Größe/SKU | Verfügbarkeit | Preis |
|---|---|---|
25 units | Warenkorb auf Verfügbarkeit prüfen | € 67,80 |
100 units | Warenkorb auf Verfügbarkeit prüfen | € 175,00 |
250 units | Warenkorb auf Verfügbarkeit prüfen | € 357,00 |
Über diesen Artikel
CAS-Nummer:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-846-3
MDL number:
EG-Nummer:
Specific activity:
≥2.0 units/mg protein
Technischer Dienst
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Unterstützung erhaltenform
lyophilized powder
Quality Segment
specific activity
≥2.0 units/mg protein
mol wt
228 kDa
composition
Protein, ≥5.0% biuret
shipped in
dry ice
storage temp.
−20°C
General description
Aldehyde dehydrogenase is a tetramer and has several different isoforms. The enzyme tested in 0.01 M pyrophosphate buffer shows a sharp optimum around pH 9.3 with acetaldehyde as substrate. Potassium ions and cysteine are essential for the enzyme′s activity. Rubidium or NH4+ can be substituted for K+, and glutathione for cysteine. Lithium, Na+, and Cs+ inhibit the reaction. Aldehyde dehydrogenase is inhibited by propylurea, crotonaldehyde, n-propyl isocyanate, cyclohexyl isocyanate, 1-n-propyl-1-[(4-chlorophenyl)sulphonyl]-3-n-propylurea, and 1-methyl-1-[(4-chlorophenyl)sulphonyl]-3-n-propylurea. The enzyme may be utilized to quantitate aldehydes present in blood.
Aldehyde dehydrogenase (ALDH) is present in the nucleus, cytosol, mitochondria and endoplasmic reticulum of cells.
Application
Biochem/physiol Actions
Aldehyde dehydrogenase from baker′s yeast catalyzes the reduction of pyridine nucleotides by several aldehydes. It catalyzes the oxidation of a wide range of substrates, such as acetaldehyde, formaldehyde, propionaldehyde, n-butylaldehyde, isobutylaldehyde, n-valeraldehyde, caproaldehyde, benzaldehyde, glycoaldehyde, D-glyceraldehyde, malonic semialdehyde, and succinic aldehyde. Aldehyde dehydrogenase is used to study the production of ethanol and isobutanol. Ethanol concentration can be determined colorimentrically by monitoring the enzymatic reduction of nicotinamide adenine dinucleotide (NAD) using alcohol dehydrogenase after preremoval of aldehyde by aldehyde dehydrogenase.
Physical form
Enthält Trehalose, Kaliumphosphat und Citrat-Puffersalze, Dithiothreitol und Spuren von β-NAD und Propionsäure.
Preparation Note
This enzyme can be dissolved at 0.3 mg/mL in 100 mM Tris-HCl buffer (pH 8.0), containing 0.02% BSA.
Other Notes
1 U oxidiert 1.0 μmol Acetaldehyd zu Essigsäure pro Minute bei 25 °C und pH 8.0 in Anwesenheit von β-NAD+, Kalium und Thiolen.
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| description lyophilized powder, ≥2.0 units/mg protein | description Type XV, lyophilized powder, 200-400 units/mg protein (modified Warburg-Christian) | description Type IX, lyophilized powder, 200-400 units/mg protein (modified Warburg-Christian) | description ammonium sulfate suspension, 100-300 units/mg protein (biuret) |
| specific activity ≥2.0 units/mg protein | specific activity 200-400 units/mg protein (modified Warburg-Christian) | specific activity 200-400 units/mg protein (modified Warburg-Christian) | specific activity 100-300 units/mg protein (biuret) |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form ammonium sulfate suspension |
| mol wt 228 kDa | mol wt 128 kDa | mol wt 128 kDa | mol wt 118 kDa |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 300 | Quality Level 200 | Quality Level 200 |
Lagerklasse
11 - Combustible Solids
Was ist los?
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
PSA (Persönliche Schutzausrüstung)
Eyeshields, Gloves, type N95 (US)
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Re A6338...how was the enzyme purified, and is it confirmed to be free of other activities, e.g. alcohol dehydrogenase?
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