The specific site of biotin attachment on the IgG molecule has not been determined.
Additionally, the biotinylation procedure is proprietary and, therefore, cannot be disclosed.
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B9183
Monoklonaler ANTI-HA-Antikörper
clone HA-7, purified from hybridoma cell culture
Aktionsangebot verfügbar
Synonym(e):
Anti-HA, Anti-Influenza-Hämagglutinin
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| Größe/SKU | Verfügbarkeit | Preis |
|---|---|---|
100 μg | Warenkorb auf Verfügbarkeit prüfen | € 449,00 |
Über diesen Artikel
Conjugate:
biotin conjugate
Clone:
HA-7, monoclonal
Application:
WB CL
Citations:
7
€ 449,00
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Technischer Dienst
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Unterstützung erhaltenbiological source
mouse
Quality Segment
conjugate
biotin conjugate
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
HA-7, monoclonal
form
buffered aqueous solution
concentration
~1 mg/mL
technique(s)
western blot (chemiluminescent): 0.25-0.50 μg/mL using HA tagged fusion proteins in transiently transfected mammalian cell extracts.
shipped in
dry ice
storage temp.
2-8°C
General description
The Monoclonal Anti-HA Biotin Conjugate antibody recognizes the HA tag sequence on the HA tagged fusion proteins when expressed at the N or C terminal to the fusion protein. This antibody is derived from the HA-7 hybridoma, which is produced from the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to the amino acid residues 98-106 (YPYDVPDYA) of human Influenza virus hemagglutinin (HA) known as HA-tag, conjugated to KLH. Ascites fluid collected from the HA-7 hybridoma is then conjugated to biotin.
Immunogen
An KLH konjugiertes synthetisches Peptid, das einem Fragment des Hämagglutinins (HA) des menschlichen Grippevirus, genannt HA-Tag, entspricht.
Application
Antibody suitable for immunoblotting.
Biochem/physiol Actions
Der Antikörper erkennt native sowie durch Denaturierung reduzierte Formen des HA-getaggten Proteins und reagiert mit am N- oder C-Endpunkt HA-getaggten Fusionsproteinen, die in E. coli oder in Säugetierzellen exprimiert werden.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| description clone HA-7, purified from hybridoma cell culture | description ~1 mg/mL, clone HA-7, purified from hybridoma cell culture | description clone HA-7, purified from hybridoma cell culture | description clone HA-7, ascites fluid |
| conjugate biotin conjugate | conjugate TRITC conjugate | conjugate unconjugated | conjugate unconjugated |
| clone HA-7, monoclonal | clone HA-7, monoclonal | clone HA-7, monoclonal | clone HA-7, monoclonal |
| antibody form purified from hybridoma cell culture | antibody form purified from hybridoma cell culture | antibody form purified immunoglobulin | antibody form ascites fluid |
| biological source mouse | biological source mouse | biological source mouse | biological source mouse |
| form buffered aqueous solution | form buffered aqueous solution | form - | form - |
| technique(s) western blot (chemiluminescent): 0.25-0.50 μg/mL using HA tagged fusion proteins in transiently transfected mammalian cell extracts. | technique(s) direct immunofluorescence: 10-15 mg/mL using mammalian cells expressing HA-tagged fusion proteins | technique(s) western blot: 0.25-1 μg/mL, immunoprecipitation (IP): 0.5-2.0 μg, immunocytochemistry: 1.0-2.0 μg/mL | technique(s) indirect ELISA: suitable, immunoprecipitation (IP): suitable, western blot: 1:20,000 using extracts from 293T (human embryonic kidney) cells expressing a recombinant HA-fusion protein, immunocytochemistry: suitable |
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Lagerklasse
10 - Combustible liquids
Was ist los?
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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Where is the biotin located on the IgG? Additionally, what protocol or method did you follow to biotinylate it?
1 answer-
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What is the isotype and light chain for Monoclonal Anti-HA antibody clone HA-7 produced in mouse?
1 answer-
Monoclonal Anti-HA antibody clone HA-7 is mouse IgG1, kappa light chain.
Helpful?
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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