C0543

Mouse Collagen Type IV

from Engelbreth-Holm-Swarm murine sarcoma basement membrane, lyophilized powder, suitable for cell culture, used for 3D hydrogel formation

• Synonym(e): Type IV collagen, EHS-collagen
• CAS-Nummer: 9007-34-5
• EG-Nummer: 232-697-4
• MDL-Nummer: MFCD00130825
• UNSPSC-Code: 12352202
• NACRES: NA.75

Eigenschaften

• Produktname: Kollagen aus Engelbreth-Holm-Swarm murine sarcoma basement membrane, Type IV (Miller), lyophilized powder, BioReagent, suitable for cell culture
• Biologische Quelle: mouse (Engelbreth-Holm-Swarm murine sarcoma basement membrane)
• Qualitätsniveau: 200
• Typ: Type IV (Miller)
• Produktlinie: BioReagent
• Form: lyophilized powder
• Verpackung: glass bottle of 1 vial
• Methode(n): cell culture | mammalian: suitable
• Oberflächendeckung: 6‑10 μg/cm²
• Löslichkeit: 0.25% acetic acid: 0.5-2.0 mg/mL (Keep at 2-8 °C for several hours, occasionally swirling.)
• UniProt-Hinterlegungsnummer: B1AVK5; P02463; P08122; Q63ZW6; Q9QZS0
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Angaben zum Gen: mouse ... Col4a1(12826), Col4a2(12827), Col4a3(12828), Col4a5(12830), Col4a6(94216)

Beschreibung

Allgemeine Beschreibung

Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.

Collagen is classified into several structurally and genetically distinct types. Type IV collagen is a predominant structural protein of the basement membrane. It is notably characteristic of the Engelbreth-Holm-Swarm (EHS) mouse sarcoma. It is commercially available as matrigel and cultrex. Matrigel is made of type 4 collagen, laminin, heparan sulfate proteoglycan and entactin obtained from engelbreth-holm-swarm murine sarcoma.[1] [2]The target cells for attachment include: epithelial cells, endothelial cells, muscle cells, and nerve cells.

Anwendung

Collagen from Engelbreth-Holm-Swarm murine sarcoma basement membrane has been used:

• in adhesion assays.[3]
• in hydrogel fabrication.[4]
• to coat cell culture plates for cell differentiation.[5]
• to treat round plastic coverslips for scanning electron microscopy (SEM) analysis in an interaction study between A. culbertsoni trophozoites and Schwann cells.[6]

Recommended for use as a cell culture substratum at 6-10 μg/cm².

Biochem./physiol. Wirkung

Collagen isolated from Engelbreth-Holm-Swarm murine sarcoma basement membrane helps in the development of 3D nodules.[1] It is also used in 2D coatings.[7]

Angaben zur Herstellung

0.75 mg/vial

Chloroform treated.
Pepsin is not used in the preparation.

Sonstige Hinweise

Native collagen

We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma-type designations with recognized collagen classification types.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)