C0543

Mouse Collagen Type IV

from Engelbreth-Holm-Swarm murine sarcoma basement membrane, lyophilized powder, suitable for cell culture, used for 3D hydrogel formation

• Synonym(e): Type IV collagen, EHS-collagen
• CAS-Nummer: 9007-34-5
• UNSPSC Code: 12352202
• NACRES: NA.75
• EC Number: 232-697-4
• MDL number: MFCD00130825
• Biological source: mouse (Engelbreth-Holm-Swarm murine sarcoma basement membrane)
• Form: lyophilized powder
• Technique(s): cell culture | mammalian: suitable

Eigenschaften

• Produktname: Kollagen aus Engelbreth-Holm-Swarm murine sarcoma basement membrane, Type IV (Miller), lyophilized powder, BioReagent, suitable for cell culture
• biological source: mouse (Engelbreth-Holm-Swarm murine sarcoma basement membrane)
• Quality Segment: 200
• type: Type IV (Miller)
• product line: BioReagent
• form: lyophilized powder
• packaging: glass bottle of 1 vial
• technique(s): cell culture | mammalian: suitable
• surface coverage: 6‑10 μg/cm²
• solubility: 0.25% acetic acid: 0.5-2.0 mg/mL (Keep at 2-8 °C for several hours, occasionally swirling.)
• UniProt accession no.: B1AVK5, P02463, P08122, Q63ZW6, Q9QZS0
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: mouse ... Col4a1(12826), Col4a2(12827), Col4a3(12828), Col4a5(12830), Col4a6(94216)

Beschreibung

General description

Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.

Collagen is classified into several structurally and genetically distinct types. Type IV collagen is a predominant structural protein of the basement membrane. It is notably characteristic of the Engelbreth-Holm-Swarm (EHS) mouse sarcoma. It is commercially available as matrigel and cultrex. Matrigel is made of type 4 collagen, laminin, heparan sulfate proteoglycan and entactin obtained from engelbreth-holm-swarm murine sarcoma. The target cells for attachment include: epithelial cells, endothelial cells, muscle cells, and nerve cells.

Application

Recommended for use as a cell culture substratum at 6-10 μg/cm².

Collagen from Engelbreth-Holm-Swarm murine sarcoma basement membrane has been used:

• in adhesion assays.
• in hydrogel fabrication.
• to coat cell culture plates for cell differentiation.
• to treat round plastic coverslips for scanning electron microscopy (SEM) analysis in an interaction study between A. culbertsoni trophozoites and Schwann cells.

Biochem/physiol Actions

Collagen isolated from Engelbreth-Holm-Swarm murine sarcoma basement membrane helps in the development of 3D nodules. It is also used in 2D coatings.

Preparation Note

0.75 mg/vial

Chloroform treated.
Pepsin is not used in the preparation.

Other Notes

Native collagen

We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma-type designations with recognized collagen classification types.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• Was ist los?: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• PSA (Persönliche Schutzausrüstung): Eyeshields, Gloves, type N95 (US)