C-12533

Human Aortic Smooth Muscle Cells (HAoSMC)

500,000 cryopreserved cells

• Synonym(e): Aortic Smooth Muscle Cells, Human Smooth Muscle Cells
• NACRES: NA.81
• UNSPSC Code: 41106514
• Biological source: human aorta (smooth muscles)
• Morphology: (smooth muscle)
• Relevant disease(s): hypertension; cardiovascular diseases

Eigenschaften

• biological source: human aorta (smooth muscles)
• product line: PromoCell^®
• packaging: pkg of 500,000 cells
• morphology: (smooth muscle)
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): hypertension; cardiovascular diseases
• shipped in: dry ice
• storage temp.: −196°C

Beschreibung

General description

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.
Our HAoSMC are now also available from HLA-typed and Diabetes Type I and Type II donors.

Application

Primary Human Aortic Smooth Muscle Cells (HAoSMC) are isolated from plaque-free regions of the human aorta and are provided in a cryopreserved format. HAoSMC stain positive for smooth muscle α-actin. The pulsatile pressure produced by the heart causes a cyclic distention of the aorta. The smooth muscle cells in the aortic wall subsequently contract it again. Changes in the arterial wall, associated with vascular diseases such as atherosclerosis and hypertension, strongly influence this process. HAoSMC are suitable for studying the role of smooth muscle cells under normal or disease conditions in vitro.

Biochem/physiol Actions

Aorta

Preparation Note

Click here for more information.

Analysis Note

Rigid quality control tests are performed for each lot of Human Smooth Muscle Cells. They are tested for cell morphology, adherence rate, and cell viability. Flow cytometric analyses for cell-type specific markers, e.g. smooth muscle a-actin, are carried out for each lot. Growth performance is tested through multiple passages up to 15 population doublings (PD) under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Other Notes

Recommended Plating Density: 7500 - 10000 cells per cm²Passage After Thawing: P2Tested Markers: Smooth muscle alpha-actin positiveGuaranteed population doublings: >15

Recommended Primary Cell Culture Media:Link

Legal Information

PromoCell is a registered trademark of PromoCell GmbH

Disclaimer

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

This product is regulated in France when intended for scientific purposes, including import, export, and conservation activities (Articles L.1211-1, paragraph 2, and L.1243-4 of the French Public Health Code). The purchaser (i.e. end user) is required to obtain an import authorization from the France Ministry responsible for Research (as referenced in Article L.1245-5-1 II of the French Public Health Code) or a conservation authorization from the same ministry (as referenced in Article L.1243-4 of the French Public Health Code). By placing an order for this product, you confirm that you have obtained the appropriate import or conservation authorization for the product. For more information regarding the use of human biological samples for research purposes in France, please contact the relevant French authorities.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable