C-12565

Human Tracheal Smooth Muscle Cells (HTSMC)

500,000 cryopreserved cells

• NACRES: NA.81
• UNSPSC Code: 41106514
• Biological source: human trachea
• Morphology: (smooth muscle)
• Relevant disease(s): asthma

Eigenschaften

• biological source: human trachea
• product line: PromoCell^®
• packaging: pkg of 500,000 cells
• morphology: (smooth muscle)
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): asthma
• shipped in: dry ice
• storage temp.: −196°C

Beschreibung

General description

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Application

Primary Human Tracheal Smooth Muscle Cells (HTSMC) are isolated from the trachea from single donors and are provided in a cryopreserved format. HTSMC stain positive for smooth muscle α-actin. They connect the endings of the tracheal cartilage by a contractile bridge called the paries membranaceus and consequently regulate the airway lumen. Under pathological conditions like asthma, the amount of smooth muscle cells in the trachea walls is increased leading to smooth muscle hypertrophy or hyperplasia. Hence, HTSMC are suitable for in vitro studies of asthma and other pulmonary diseases.

Biochem/physiol Actions

Trachea

Preparation Note

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Analysis Note

Rigid quality control tests are performed for each lot of Human Smooth Muscle Cells. They are tested for cell morphology, adherence rate, and cell viability. Flow cytometric analyses for cell-type specific markers, e.g. smooth muscle a-actin, are carried out for each lot. Growth performance is tested through multiple passages up to 15 population doublings (PD) under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Other Notes

Recommended Plating Density: 7500 - 10000 cells per cm²Passage After Thawing: P2Tested Markers: Smooth muscle alpha-actin positiveGuaranteed population doublings: >15

Recommended Primary Cell Culture Media:Link

Legal Information

PromoCell is a registered trademark of PromoCell GmbH

Disclaimer

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• Was ist los?: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable