C-12732

Human White Preadipocytes (HWP)

Visceral, 500,000 cryopreserved cells

• NACRES: NA.81
• UNSPSC Code: 41106514
• eCl@ss: 32040503
• Biological source: human adipose tissue (subcutaneous or visceral)
• Morphology: (adipocytes)

Eigenschaften

• biological source: human adipose tissue (subcutaneous or visceral)
• product line: PromoCell^®
• packaging: pkg of 500,000 cells
• morphology: (adipocytes)
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Beschreibung

General description

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

Application

Primary Human White Preadipocytes (HWP) are isolated from adult subcutaneous or visceral adipose tissue from different locations (lot specific source information is available on request) and provided in a cryopreserved format.Adipose tissue is crucial in energy storage and metabolic homeostasis. An increase in adipose tissue results either from an enlargement of mature adipocytes or from the differentiation of adipocyte precursor cells (preadipocytes) into new mature adipocytes. These preadipocytes are already present in adipose tissues. They can proliferate throughout adult life and replace the cells that have differentiated into mature adipocytes. Since this differentiation process is controlled by a variety of growth factors and hormones, preadipocytes are suitable for the investigation of the physiological mechanisms controlling proliferation, differentiation, and function of adipose tissue.

Biochem/physiol Actions

Adipose Tissue

Preparation Note

Click here for more information.

Analysis Note

Rigid quality control tests are performed for each lot of Human White Preadipocytes. They are tested for cell morphology, adherence rate, and cell viability. Growth performance is tested through multiple passages up to 10 population doublings (PD) under culture conditions without antibiotics and antimycotics. Furthermore, each lot of Human White Preadipocytes is extensively tested for its capacity to differentiate into mature adipocytes. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Other Notes

Recommended Plating Density: 5000 cells per cm²Passage After Thawing: P2Tested Markers: Differentiation into adipocytes testedGuaranteed population doublings: >10

Recommended Primary Cell Culture Media:Link

Recommended Primary Cell Culture Media:Link

Legal Information

PromoCell is a registered trademark of PromoCell GmbH

Disclaimer

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

This product is regulated in France when intended for scientific purposes, including import, export, and conservation activities (Articles L.1211-1, paragraph 2, and L.1243-4 of the French Public Health Code). The purchaser (i.e. end user) is required to obtain an import authorization from the France Ministry responsible for Research (as referenced in Article L.1245-5-1 II of the French Public Health Code) or a conservation authorization from the same ministry (as referenced in Article L.1243-4 of the French Public Health Code). By placing an order for this product, you confirm that you have obtained the appropriate import or conservation authorization for the product. For more information regarding the use of human biological samples for research purposes in France, please contact the relevant French authorities.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable