C-12914

Human M1 Macrophages (hMDM-GMCSF(-))

Monocyte-derived, single donor, 1.5 million cryopreserved cells

• Synonym(e): hMDM-GMCSF(-) cells
• NACRES: NA.81
• UNSPSC Code: 41106514
• Biological source: human blood (monocyte)
• Morphology: (macrophages)

Eigenschaften

• biological source: human blood (monocyte)
• product line: PromoCell^®
• packaging: pkg of 1,500,000 cells
• morphology: (macrophages)
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Beschreibung

General description

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Application

Cryopreserved human monocyte-derived macrophages (hMDM) are convenient and easy-to-handle. The thawed cells plate into all tissue culture vessel formats and can be maintained as adherent, biologically functional cultures for several weeks. Optionally, user-customizable activation of the cells can be performed.Cryopreserved human macrophages are produced from monocytes in M1 Macrophage Generation Media DXF and are available as non-activated, fully qualified M1- (GM-CSF) polarized cells. After 9-10 days of differentiation, the M1 macrophages are cryopreserved using Cryo-SFM. Each cryo vial contains more than 1.5 million viable cells after thawing.

Biochem/physiol Actions

Monocyte

Preparation Note

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Other Notes

Recommended Plating Density: 100000 cells per cm²Tested Markers: CD80 positive, CD68 positiveNote: The plated cells do not proliferate. Recommended plating density for obtaining an adherent cell monolayer with 70-90% confluency.

Recommended Primary Cell Culture Media:Link

Recommended Primary Cell Culture Media:Link

Legal Information

PromoCell is a registered trademark of PromoCell GmbH

Disclaimer

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• Was ist los?: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable