C2978
Synonym(e):
Whole-cell protein extraction solution
| Größe/SKU | Verfügbarkeit | Preis |
|---|
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| description Cell Lysis Reagent, Suitable for Mammalian cell lysis and protein solubilization. | description - | description For yeast cells | description Individually packed in foil blister packs |
| form solution | form - | form solution | form tablet |
| storage temp. room temp | storage temp. −20°C | storage temp. - | storage temp. room temp |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level - |
| input mammalian sample(s) | input mammalian sample(s) | input bacteria and yeast sample(s) | input - |
12 - Non Combustible Liquids
Not applicable
Not applicable
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The formulation is proprietary and below are the information that we can share.
This product does not contain any denaturants except for the "proprietary mild detergent" and the pH of the reagent is 7.6.
The reagent contains 10 mM salt and the detergent is known to be compatible with two dimensional gel electrophoresis.
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The Product Information Sheet recommends a protease inhibitor suitable for mammalian cell and tissue extracts, such as product numbers P8340, PIC0002, or I3786, as listed under "Reagents and Equipment Required but Not Provided." After adding the CelLytic M reagent, a 15-minute incubation on a shaker is recommended. The lysate should not be vortexed.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/113/033/c2978bul-ms.pdf
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The formulation does not contain EDTA or chelators. There is no known reason for it to be incompatible with Phos-Tag gel, but it has not been tested for this specific application.
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As mentioned in the 'DESCRIPTION' section, the composition of this product is considered proprietary and may not be shared. This product does not contain any denaturants except for a proprietary mild detergent. Lysates obtained with this product may be immediately run on a SDS PAGE after the addition of loading buffer.
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Is the product compatible with the BCA assay? Additionally, are there any denaturants in the formulation?
CelLytic™ M is suitable for Bradford Assay and BCA assays. For the Lowry method, there is a background issue that is problematic for low protein concentrations, but may be subtracted with high protein concentrations.
CelLytic™ M does not contain any primary amines; it is a buffered solution (pH 7.6) which has no denaturants except for a mild detergent. The exact formulation is proprietary. This product may be diluted or dialyzed if it is found to have interference in your particular assay.
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Does the CelLytic M (Cat. No.: 2978) contain SDS, and if it does, at what concentration?
The product with the code C2978, CelLytic M, does not contain SDS. Instead, it contains a mild detergent in a buffer.
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Is the C2978 lysis buffer suitable for viral DNA extraction from cultured mammalian cells (fewer than 25,000 cells/ul) and subsequent qPCR?
The cellular DNA is centrifuged down and will not be present in the supernatant along with the proteins. To isolate the DNA, a different kit is necessary.
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What is the critical micelle concentration (CMC) of the product?
Unfortunately, the CMC value has not been tested for this product.
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We have not tested CelLytic for neutralizing HIV nor used in a BSL3 facility.
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What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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