C4985

Cytochrome P450 human

4F2 isozyme microsomes, with P450 Reductase and cytochrome b₅, recombinant, expressed in baculovirus infected insect cells (BTI-TN-5B1-4)

• UNSPSC Code: 12161501
• NACRES: NA.47
• EG-Nummer: 1.6.2.4 (BRENDA, IUBMB)
• MDL number: MFCD03456520
• Biological source: human
• Recombinant: expressed in baculovirus infected insect cells (BTI-TN-5B1-4)

Eigenschaften

• biological source: human
• Quality Segment: 200
• recombinant: expressed in baculovirus infected insect cells (BTI-TN-5B1-4)
• form: solution
• mol wt: 45-60 kDa
• packaging: vial of 0.5 nmol
• UniProt accession no.: P78329
• application(s): cell analysis
• storage temp.: −70°C
• Gene Information: human ... CYP4F2(8529)

Beschreibung

Biochem/physiol Actions

Cytochrome P450 is a heterogeneous family of isozymes whose primary function is to oxidize small molecules, both as a function of intermediary metabolism (e.g., fatty acids) and to detoxify exogenous compounds (drugs or toxins). Some isoforms have narrow substrate specificity, while others are promiscuous. The CYP1A1 isoform catalyzes 7-deethylation of ethoxyresorufin. Cytochrome P450 (CYP) plays an important role in detoxifying xenobiotics, cellular metabolism and homeostasis. One of the main mechanisms of drug-drug interactions is the induction or inhibition of these enzymes. CYP enzymes are transcriptionally activated by a variety of xenobiotics and by endogenous substrates via receptor-dependent pathways. Inhibition of these enzymes is a major factor in metabolism-based drug-drug interactions, and many chemotherapeutic medications can cause drug interactions by either inhibiting or inducing the cytochrome p450 enzyme system.

The CYP4F2 isoform catalyzes the 20-hydroxylation of leukotriene B₄.

Physical form

Solution in 100 mM potassium phosphate buffer, pH 7.4.

Other Notes

One unit will hydroxylate 1 picomole of leukotriene B4 per minute at pH 7.4 at 37°C.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable