The formulation includes EDTA and sodium citrate, which chelate or sequester divalent cations, thereby disrupting the calcium (Ca²⁺) and magnesium (Mg²⁺) bridges responsible for cell–cell adhesion.
Fortfahren mit
C5914
Zellauflösungslösung, nicht enzymatisch 1x
Prepared in phosphate buffered saline without calcium and magnesium, sterile-filtered, BioReagent, suitable for cell culture
Synonym(e):
Dissoziationslösung
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Über diesen Artikel
Concentration:
1 ×
Quality Segment
sterility
sterile-filtered
product line
BioReagent
form
liquid
concentration
1 ×
technique(s)
cell culture | mammalian: suitable
pH
6.9-7.1
shipped in
wet ice
storage temp.
2-8°C
General description
Die Zelldissoziation mit nicht-enzymatischen Methoden in den mesenchymalen Stromazellen des Menschen unterstützt die Zellablösung und erhält die Zellfunktionen, insbesondere die Expression des Chemokinrezeptors an der Zelloberfläche.
Application
Nicht-enzymatische Spezialformulierung für das behutsame Ablösen von an Kunststoff- oder Glasoberflächen haftenden Zelltypen.
Die enzymfreie Zelldissoziationslösung 1x wird bei der Dissoziation von retinalen Astrozytenkulturen, U87-Glioblastom-Tumorzellen und kardialen Stammzellen (CSC) verwendet.
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Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Lagerklasse
12 - Non Combustible Liquids
Was ist los?
WGK 2
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How does the product C5914 dissociate the cells from culture dishes etc?
1 answer-
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Can one use Product C5914, Cell Dissociation Solution, with tissue?
1 answer-
We have only used with monolayer cell culture, and therefore we have no information on its usage with tissue.
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Does Product C5914, Cell Dissociation Solution, work with epithelial cells?
1 answer-
It will work with most cell types.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Does Product C5914, Cell Dissociation Solution, contain EDTA?
1 answer-
Yes, it does contain EDTA; the concentration is proprietary.
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When trypsin is used to detach cells, one usually adds fetal bovine serum (FBS) to the media. Should FBS be used with Product C5914, Cell Dissociation Solution?
1 answer-
No. FBS is used to inactivate trypsin. This product is not enzymatic, so there is no need to use FBS. The cells can be washed with phosphate buffered saline (PBS). When the cells are to be cultured, then one would need to add FBS.
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