C8413

Chloramphenicol Acetyltransferase from Escherichia coli

buffered aqueous glycerol solution

• Synonym(e): Acetyl-CoA: chloramphenicol 3-O-acetyltransferase, CAT
• CAS-Nummer: 9040-07-7
• UNSPSC Code: 12352204
• MDL number: MFCD00130772
• EG-Nummer: 2.3.1.28 (BRENDA, IUBMB)
• Specific activity: 50,000-150,000 units/mg protein

Eigenschaften

• form: buffered aqueous glycerol solution
• specific activity: 50,000-150,000 units/mg protein
• mol wt: 75 kDa (three identical subunits)
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: Escherichia coli ... cat(2847485)

Beschreibung

Application

The enzyme has been used in chloramphenicol acetyltransferase assay to optimize the transfection of plasmid DNA into primary cultures of adult mouse keratinocytes. It has also been used to assess the acetyl-CoA carboxylase-carboxyltransferase (ACC-CT) domain activity. This has been done using a coupled-two phase system measuring the selective partition of [¹⁴C]acetylchloramphenicol into an organic layer.

Chloramphenicol acetyltransferase from Escherichia coli has been used in a study to assess the construction of a novel expression system in Klebsiella pneumoniae and its application for 1,3-propanediol production. Chloramphenicol acetyltransferase from Escherichia coli has also been used in a study to investigate site-directed mutagenesis and promoter functional analysis of the RM07 DNA fragment from Halobacterium halobium.

Biochem/physiol Actions

The enzyme responsible for chloramphenicol resistance in bacteria. Catalyzes the conversion of acetyl-CoA + chloramphenicol to CoA + chloramphenicol 3-acetate.

Physical form

Clear, colorless solution in 50% glycerol containing 5 mM Tris-HCl, pH 7.8, and 0.5 mM 2-mercaptoethanol

Other Notes

One unit will convert 1.0 nanomole of chloramphenicol and acetyl-CoA to chloramphenicol 3-acetate and CoA per min at pH 7.8 at 25 °C.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• Was ist los?: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable