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C8667

Cholesterin

Sigma Grade, ≥99%

Cholesterol
1 of 1 reviewers received a sample product or took part in a promotion

Synonym(e):

3β-Hydroxy-5-cholesten, 5-Cholesten-3β-ol

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Ihnen/SKUVerfügbarkeitPreis
500 mg
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€ 39,30
1 g
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€ 49,70
5 g
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€ 85,60
25 g
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€ 279,00
100 g
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€ 891,00

Über diesen Artikel

Empirische Formel (Hill-System):
C27H46O
CAS-Nummer:
57-88-5
Molekulargewicht:
386.65
NACRES:
NA.77
PubChem Substance ID:
24893094
eCl@ss:
39023139
UNSPSC Code:
12352111
EC Number:
200-353-2
MDL number:
MFCD00003646
Beilstein/REAXYS Number:
1915888

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Unterstützung erhalten

biological source

sheep wool

Quality Segment

200

grade

Sigma Grade

assay

≥99%

form

powder

bp

360 °C (lit.)

mp

147-149 °C (lit.)

solubility

water: soluble 0.00003 g/L at 20 °C

density

1.067 g/mL at 25 °C (lit.)

application(s)

advanced drug delivery

shipped in

ambient

storage temp.

−20°C

SMILES string

CC(C)CCC[C@@H](C)[C@H]1CC[C@H]2[C@@H]3CC=C4C[C@@H](O)CC[C@]4(C)[C@H]3CC[C@]12C

InChI

1S/C27H46O/c1-18(2)7-6-8-19(3)23-11-12-24-22-10-9-20-17-21(28)13-15-26(20,4)25(22)14-16-27(23,24)5/h9,18-19,21-25,28H,6-8,10-17H2,1-5H3/t19-,21+,22+,23-,24+,25+,26+,27-/m1/s1

InChI key

HVYWMOMLDIMFJA-DPAQBDIFSA-N

Gene Information

human ... POLA1(5422), TOP2A(7153)
rat ... Ar(24208), Polb(29240)

Application

Standard für die Chromatographie
Cholesterin wurde für die Präparation von immunstimulierenden Komplexen verwendet, um die zellulären und humoralen Immunantworten zu verstärken.[1] Es wurde Tierfutter beigemischt, um die Auswirkungen einer cholesterinreichen Ernährungsweise zu untersuchen.

Biochem/physiol Actions

Cholesterin ist ein Lipid, das zwischen 20 und 25 % der Strukturkomponenten in Zellmembranen ausmacht. Es bestimmt die Fluidität und Durchlässigkeit der Membran und macht sie für Wasser durchlässig, nicht aber für Ionen und Protonen. Cholesterin reguliert die Transportfunktionen und signalisiert das Vorhandensein von Proteinen auf der Plasmamembran.[2] Die Cholesterinsynthese erfolgt hauptsächlich im Dünndarm und in der Leber.[3]
Hauptbestandteil biologischer Membranen; ca. 25% des gesamten Lipids im Gehirn ist Cholesterin.

Preparation Note

Cholesterin-Monohydrat bildet in heißem Alkohol eine klare, farblose bis leicht gelbliche Lösung. Es ist auch in Aceton, Dioxan, Ethylacetat, Benzol, Petrolether, Ölen, Fetten und in wässrigen Gallensalzlösungen löslich. Die Lösungen sollten vor Licht geschützt aufbewahrt werden.

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1 of 1

Dieser Artikel
26732NIST911CPHR1533
Cholesterin Sigma Grade, ≥99%

Sigma-Aldrich

C8667

Cholesterin

Cholesterin from lanolin, ≥99.0% (GC)

Sigma-Aldrich

26732

Cholesterin

Cholesterin NIST® SRM® 911c

NIST911C

Cholesterin

Cholesterin Pharmaceutical Secondary Standard; Certified Reference Material

Supelco

PHR1533

Cholesterin

description

Sigma Grade, ≥99%

description

from lanolin, ≥99.0% (GC)

description

NIST® SRM® 911c

description

Pharmaceutical Secondary Standard; Certified Reference Material

assay

≥99%

assay

≥99.0% (GC)

assay

-

assay

-

biological source

sheep wool

biological source

lanolin

biological source

-

biological source

-

grade

Sigma Grade

grade

-

grade

certified reference material

grade

certified reference material, pharmaceutical secondary standard

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

-

storage temp.

-10 to -25°C

shipped in

ambient

shipped in

-

shipped in

-

shipped in

-

form

powder

form

powder

form

-

form

powder

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Lagerklasse

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Analysenzertifikate (COA)

Lot/Batch Number

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Questions

1–7 of 7 Questions  

  1. Hello, I would like to know how to dissolve this product in serum, and what its maximum solubility in serum is? Because I am looking for a cholesterol product to prepare a solution with a concentration >250 mg/dL. Thank you!

    1 answer

    1. Free cholesterol is practically insoluble in serum. A true solution at a concentration of 250 mg/dL cannot be reliably achieved. Typical cholesterol levels in human serum may range from 150 – 250 mg/dL, with 30 – 70 mg/dL as free cholesterol. The majority is bound to lipoproteins in the serum matrix. Higher concentrations may be possible with heat, however this has not been investigated. The end user would have to determine the rate. Please see the link below to review a water-soluble cholesterol formulation, C4951. This product is a cyclodextrin complex that enhances cholesterol solubility up to 300 mg/mL (approximately 50 mg cholesterol).
      https://www.sigmaaldrich.com/product/sigma/c4951

      Please see the link below to review additional information regarding cholesterol transport in serum:
      https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/research-and-disease-areas/metabolism-research/cholesterol-transport

      Helpful?

  2. how to prepare for cell culture? thank you

    1 answer

    1. Here is the link to our Biochemical Solubility table:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/287/917/c3045inf.pdf
      For the Cell Culture Tested grade of this product, C3045, three options are provided: Serum, Tween and Ethanol.
      Note that if Serum is used as the solvent, it may already contain some Cholesterol. The "Tween" is referring to an aqueous solution of either TWEEN 20 or TWEEN 80. Also, Ethanol can be used to make a stock solution, before supplementing the culture medium; consider perhaps a 100X solution in ethanol, which would give a concentration of 1 percent ethanol in the medium.

      Helpful?

  3. What is the best temperature for storage for 5 mg/mL solutions in ethanol? Can they be stored at 4C? Thank you!

    1 answer

    1. The stability of this material in solution at 2-8°C has not been evaluated. Solutions prepared in Chloroform, in aliquots and protected from light, are stable for at least 1 year when stored at -20°C. Solutions prepared in Ethanol are expected to be similarly stable.

      Helpful?

  4. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  5. Buongiorno, volevo chiedere cortesemente quale sia l'origine del prodotto

    1 answer

    1. This product is derived from sheep wool. The origin information of this product will be listed on the lot-specific Certificate of Origin. Please navigate to the ‘DOCUMENTATION’ section of the Product Detail Page to access a Certificate under the ‘Certificate of Origin’ tab:
      https://www.sigmaaldrich.com/product/sigma/c8667#product-documentation

      Helpful?

  6. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  7. Hi, I want to know if this is from plant or sheep wool?

    1 answer

    1. As mentioned in the PROPERTIES section of the product page, this material is from sheep wool.

      Helpful?

1 Review This action will navigate to reviews.
1 of 1 reviewers received a sample product or took part in a promotion

Reviews

Active Filters

    • Review 1
    • Vote 1
    3 out of 5 stars.

    Cholesterol

    Used as standard for TLC, worked OK, stainned less than other lipids with iodine, but that may be be normal

    Helpful?

    1. Response from Merck KGaA:

      Thank you for taking the time to leave a review! We would like to learn more about your experience to see if there is anything we can do to help correct this issue. When you have a moment, please contact us by visiting https://www.sigmaaldrich.com/support/customer-support and submit a Product Technical Inquiry. We look forward to hearing from you soon!