Fortfahren mit
C9409
Creatinine Deiminase microbial
lyophilized powder, ≥25 units/mg protein
Synonym(e):
Creatinine Deaminase
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| Größe/SKU | Verfügbarkeit | Preis |
|---|---|---|
250 units | Warenkorb auf Verfügbarkeit prüfen | € 950,00 |
Über diesen Artikel
CAS-Nummer:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
253-449-1
MDL number:
EG-Nummer:
Specific activity:
≥25 units/mg protein
€ 950,00
Warenkorb auf Verfügbarkeit prüfen
Technischer Dienst
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Unterstützung erhaltenform
lyophilized powder
Quality Segment
specific activity
≥25 units/mg protein
mol wt
~260 kDa
composition
Protein, ≥15% biuret
storage temp.
−20°C
General description
Isoelectric point : 4.4
Michaelis constant : 3.5 x 10‾3M (Creatinine)
Structure : 6 subunits per mol of enzyme
Inhibitors : Ag+,Hg++, o-phenanthroline,monoiodoacetate
Optimum pH : 8.5 – 9.5
Optimum temperature : 65 – 75°C
pH Stability : pH 7.0 – 11.0 (30°C, 20hr)
Thermal stability : Below 65°C (pH 7.5, 1hr)
Michaelis constant : 3.5 x 10‾3M (Creatinine)
Structure : 6 subunits per mol of enzyme
Inhibitors : Ag+,Hg++, o-phenanthroline,monoiodoacetate
Optimum pH : 8.5 – 9.5
Optimum temperature : 65 – 75°C
pH Stability : pH 7.0 – 11.0 (30°C, 20hr)
Thermal stability : Below 65°C (pH 7.5, 1hr)
Application
Creatinine Deiminase microbial has been used:
- to immobilize aminosilylated glass beads based biosensor for ammonia/ammonium and creatinine detection in urine
- in creating creatinine-sensing membrane for biophysical studies
- to investigate the bioelectronic tongue for the simultaneous determination of urea, creatinine and alkaline ions in clinical samples
Creatinine deiminase has been used in a study to assess the application of a creatinine-sensitive biosensor for hemodialysis control. Creatinine deiminase has also been used in a study to investigate the bioelectronic tongue for the simultaneous determination of urea, creatinine and alkaline ions in clinical samples.
Biochem/physiol Actions
Physical form
Lyophilized powder containing mannitol as stabilizer
Other Notes
One unit will hydrolyze 1.0 μmole of creatinine to N-methylhydantoin and NH3 per min at pH 7.5 at 37 °C in a coupled system with L-glutamic dehydrogenase.
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Dieser Artikel | |||
|---|---|---|---|
| description lyophilized powder, ≥25 units/mg protein | description Pharmaceutical Secondary Standard; Certified Reference Material | description NIST®SRM® | description 98 atom % D, 98 atom % 15N, 99 atom % 13C, 97% (CP) |
| specific activity ≥25 units/mg protein | specific activity - | specific activity - | specific activity - |
| form lyophilized powder | form - | form - | form solid |
| mol wt ~260 kDa | mol wt - | mol wt - | mol wt - |
| storage temp. −20°C | storage temp. 2-30°C | storage temp. −70°C | storage temp. - |
| Quality Level 200 | Quality Level 300 | Quality Level - | Quality Level - |
| composition Protein, ≥15% biuret | composition - | composition - | composition - |
Lagerklasse
11 - Combustible Solids
Was ist los?
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
PSA (Persönliche Schutzausrüstung)
Eyeshields, Gloves, type N95 (US)
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