LA-N-2

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 6041202, human nerve, Large elongated cells + small round cells in clusters

• UNSPSC Code: 41106514
• Biological source: human nerve
• Relevant disease(s): cancer
• Growth mode: Not specified
• Karyotype: Modal no. 73, range 46 - 73
• Morphology: Large elongated cells + small round cells in clusters
• Manufacturer/tradename: ECACC

Eigenschaften

• biological source: human nerve
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 06041202-DNA-5UG, pkg of vial of cells 06041202-1VL
• growth mode: Not specified
• karyotype: Modal no. 73, range 46 - 73
• morphology: Large elongated cells + small round cells in clusters
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Beschreibung

Biochem/physiol Actions

Established by Seeger et al., (1977) from the primary site of a noncatecholamine-producing neuroblastoma of a 3-year-old female with clinical Stage IV neuroblastoma. The culture consists mostly of large, elongated cells with processes with some small round cells adhering to the elongated cells. The cells are tumourigenic in nude mice.

Human Neuroblastoma

Preparation Note

EMEM (with non-essential amino acids) and Ham′s F12 (1:1 mixture) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

For routine maintenance, split cultures after they have become dense i.e. once every 3-4 weeks at a 1:20 to 1:50 ratio; 8% CO₂; 37oC. The culture consists of large elongated cells with processes and small round cells. The latter tend to grow in dense clusters on the former and detach readily. Most cells are moderately adherent. Remove attached cells from the substrate with trypsin/EDTA. Cells will detach in 5-10 minutes. When resuscitated from a frozen ampoule cells may appear dead after a day but reattach and resume growth within 2-3 days. Cells grow best and are most adherent on a plastic substrate in medium at a pH of 6.9 - 7.2; they do not tolerate more alkaline pH well. Cultures hold well at high density with periodic medium changes.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.