T47D Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85102201, human breast, Epithelial

• Synonym(e): T-47-D Cells, T-47D Cells, T47D:A Cells
• UNSPSC Code: 41106514
• Biological source: human breast
• Relevant disease(s): cancer
• Growth mode: Adherent
• Karyotype: 2n = 46, hypertriploid, modal no. 65
• Morphology: Epithelial
• Manufacturer/tradename: ECACC

Eigenschaften

• biological source: human breast
• description: Human breast tumor
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 85102201-DNA-5UG, pkg of vial of cells 85102201-1VL
• growth mode: Adherent
• karyotype: 2n = 46, hypertriploid, modal no. 65
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Beschreibung

Application

T47D cell line human has been used:

• to study the effects of a polysaccharide–protein complex (PPC) isolated from metabolites of gut bacteria Raoultella ornithinolytica from the earthworm Dendrobaena veneta against Candida albicans
• has been cultured and incubated with bone marrow-derived macrophages to study their migration and podosome formation
• to study the anti-proliferative effects of two novel classes of potential antimetabolites on T47D cells

Biochem/physiol Actions

Established from the pleural effusion of a ductal carcinoma of the breast of a 54-year-old female. The cells carry receptors for a variety of steroids.

Human breast tumour

STR-PCR Data: Amelogenin: X
CSF1PO: 11,13
D13S317: 12
D16S539: 10
D5S818: 12
D7S820: 11
THO1: 6
TPOX: 11
vWA: 14

Preparation Note

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.