SK.N.SH Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 86012802, human nerve (bone marrow metastasis; Caucasian neuroblastoma), Epithelial

• Synonym(e): NSH Cells, SK-NSH Cells, SKNSH Cells
• UNSPSC Code: 41106514
• Biological source: human nerve (bone marrow metastasis; Caucasian neuroblastoma)
• Relevant disease(s): metastasis
• Growth mode: Adherent
• Karyotype: 2n = 46, modal no. 47
• Morphology: Epithelial
• Manufacturer/tradename: ECACC

Eigenschaften

• biological source: human nerve (bone marrow metastasis; Caucasian neuroblastoma)
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 86012802-DNA-5UG, pkg of vial of cells 86012802-1VL
• growth mode: Adherent
• karyotype: 2n = 46, modal no. 47
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): metastasis
• shipped in: dry ice
• storage temp.: −196°C

Beschreibung

Application

Human SK.N.SH cell line has been used in dark toxicity testing of a chloro-indium-phthalocyanine photosensitizer (In-Pc). It has also been used to study the potential mechanisms and domain requirements of the hHSJ1a (DnaJ heat shock protein family member B2) mediated reduction in superoxide dismutase 1 (SOD1) aggregation.

Biochem/physiol Actions

Established from a bone marrow metastasis from a 4-year-old caucasian female suffering with neuroblastoma. The cells have been used as a target cell in cell-mediated cytoxicity tests.

Human Caucasian neuroblastoma

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 11
D16S539: 8,13
D5S818: 12
D7S820: 7,10
THO1: 7,10
TPOX: 8,11
vWA: 14,18

Preparation Note

EMEM (EBSS) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.