RPMI 8226 Human Blood, ECACC 87012702

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 87012702, human blood, Not specified

• Synonym(e): RPMI8226 Cells, Roswell Park Memorial Institute 8226 Cells
• UNSPSC Code: 41106514
• eCl@ss: 32040503
• Biological source: human blood
• Relevant disease(s): cancer
• Growth mode: Suspension
• Karyotype: Not specified
• Morphology: Not specified
• Manufacturer/tradename: ECACC

Eigenschaften

• Produktname: RPMI 8226, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 87012702, human blood, Not specified
• biological source: human blood
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 87012702-DNA-5UG, pkg of vial of cells 87012702-1VL
• growth mode: Suspension
• karyotype: Not specified
• morphology: Not specified
• products: Immunoglobulin G (Ig) lambda light chain
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Beschreibung

Application

RPMI 8226 has been used:

• to grow human myeloma cell lines
• to evaluate the effect of 1-aryl-2-methylthioimidazolines (IIV) on the viability of RPMI 8226 cells
• to measure cytokine production by bone marrow stromal cells (BMSCs) after contact with the RPMI 8226 cell line

Biochem/physiol Actions

Derived from the peripheral blood of a 61 year old male with multiple myeloma. The cells produce and secrete Ig lambda light chain.

Human myeloma

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 12
D13S317: 11
D16S539: 9
D5S818: 11,13
D7S820: 9,10
THO1: 8
TPOX: 8,11
vWA: 16,18

Preparation Note

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. After resuscitation cell debris will be present in culture. This will decrease with subsequent subculture.

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.

Disclaimer

This product is regulated in France when intended for scientific purposes, including import, export, and conservation activities (Articles L.1211-1, paragraph 2, and L.1243-4 of the French Public Health Code). The purchaser (i.e. end user) is required to obtain an import authorization from the France Ministry responsible for Research (as referenced in Article L.1245-5-1 II of the French Public Health Code) or a conservation authorization from the same ministry (as referenced in Article L.1243-4 of the French Public Health Code). By placing an order for this product, you confirm that you have obtained the appropriate import or conservation authorization for the product. For more information regarding the use of human biological samples for research purposes in France, please contact the relevant French authorities.