NG115-401L Mouse x Rat Hybridoma, ECACC 87032003

87032003, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514
• Biological source: Mouse x Rat Hybridoma hybrid
• Technique(s): cell culture | mammalian: suitable
• Morphology: Glial

Eigenschaften

• Produktname: NG115-401L, 87032003, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
• biological source: Mouse x Rat Hybridoma hybrid
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 87032003-DNA-5UG, pkg of vial of cells 87032003-1VL
• growth mode: Adherent
• karyotype: Not specified
• morphology: Glial
• products: IR-substance P-like material
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Beschreibung

Application

Hybridisation and fusion partner studies

Biochem/physiol Actions

A somatic hybrid derived by the hybridisation of the clonal mouse neuroblastoma N115TG-2 and the clonal rat glioma C6BV-1. The cells produce significant amounts of IR-substance P-like material.

Mouse neuroblastoma x Rat glioma hybrid

Preparation Note

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.