BF-11 Rat x Mouse Hybridoma, ECACC 95050413

no DNA, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514
• Biological source: Rat x Mouse Hybridoma
• Technique(s): cell culture | mammalian: suitable
• Myeloma: Sp2/0 Ag-14
• Reactivity: Anti-Erythropoietin, human

Eigenschaften

• Produktname: BF-11, no DNA, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
• biological source: Rat x Mouse Hybridoma
• packaging: pkg of vial of cells 95050413-1VL, tube of 5 μg 95050413-DNA-5UG
• manufacturer/tradename: ECACC
• ImmunoDon: Rat AC1 spleen cells
• Myeloma: Sp2/0 Ag-14
• Reactivity: Anti-Erythropoietin, human
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Beschreibung

Immunogen

Human erythropoietin

Application

Immunological studies, isolation of erythropoietin by immuno-affinity methods.

The isotype of the monoclonal antibody produced by this cell line is rat IgG2a.

Biochem/physiol Actions

Rat ACI spleen x Mouse Sp2/0 Ag-14

The BF-11 hybridoma cell line secretes a rat monoclonal antibody named Epoclonalan that reacts with human erythropoietin. Spleen cells from ACI strain rats that had been immunised with human urinary erythropoietin have been fused with Sp2/0 Ag-14 to produce this cell line. The α, β and asialo forms of human erythropoietin are bound by the antibody to the same extent.

Preparation Note

DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Maintain cultures between 100,000 - 1,000,000 cells/ml; 5% CO₂; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Genomic DNA is not available for this cell line. Use of this product by commercial organizations may require a license agreement. Commercial organizations will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of this cell line is prohibited without the express written permission of UKHSA. Inquiries regarding any other authorized use of this cell line may be directed to culturecollections@ukhsa.gov.uk.