D2196

DAF-FM solution

5 mM in DMSO, ≥97% (HPLC)

• Synonym(e): 4-Amino-5-methylamino- 2′,7′-difluorofluorescein, Diaminofluorescein-FM
• Empirische Formel (Hill-System): C₂₁H₁₄F₂N₂O₅
• CAS-Nummer: 254109-20-1
• Molekulargewicht: 412.34
• MDL number: MFCD29073049
• UNSPSC Code: 12352200
• NACRES: NA.77
• Assay: ≥97% (HPLC)
• Form: DMSO solution
• Storage condition: desiccated

Eigenschaften

• Quality Segment: 100
• assay: ≥97% (HPLC)
• form: DMSO solution
• storage condition: desiccated
• color: colorless to yellow, including pink
• shipped in: wet ice
• storage temp.: −20°C
• SMILES string: Fc1cc2c(cc1O)Oc3c(cc(c(c3)O)F)C42OC(=O)c5c4ccc(c5N)NC
• InChI: 1S/C21H14F2N2O5/c1-25-13-3-2-8-18(19(13)24)20(28)30-21(8)9-4-11(22)14(26)6-16(9)29-17-7-15(27)12(23)5-10(17)21/h2-7,25-27H,24H2,1H3
• InChI key: DIJCILWNOLHJCG-UHFFFAOYSA-N

Beschreibung

Application

DAF-FM solution has been used as a fluorescent probe for detecting nitric oxide (NO) from plant samples.

Biochem/physiol Actions

DAF-FM is a highly sensitive, photo-stable fluorescent probe for the detection of nitric oxide (NO).

DAF-FM is a highly sensitive, photo-stable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM is more sensitive to nitric oxide (NO) compared with DAF-2. DAF-FM (Ex: 500 nm; Em: 515 nm; dectection limit: 3 nM) is more photo-stable and less pH sensitive than DAF-2. DAF-FM is not quite as cell-permeable as the diacetate derivative, but it does not need to be activated by cytosolic enzymes.

Features and Benefits

This compound is featured on the Nitric Oxide Synthases page of the Handbook of Receptor Classification and Signal Transduction. To browse other handbook pages, click here.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable