This product is a purified DNA preparation from human placenta, however this material is not tested for the presence of RNA.
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D3035
Desoxyribonukleinsäure aus menschlicher Placenta
buffered aqueous solution, sexed, female
Synonym(e):
DNA
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Über diesen Artikel
Biological source:
human placenta
Form:
buffered aqueous solution
Storage temp.:
−20°C
Preise und Verfügbarkeit sind derzeit nicht verfügbar.
Technischer Dienst
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Unterstützung erhaltenbiological source
human placenta
Quality Segment
grade
Molecular Biology
form
buffered aqueous solution
shipped in
dry ice
storage temp.
−20°C
SMILES string
[P](=O)(OC2C(OC(C2)N)CO[P](=O)(OC3C(OC(C3)N)CO)O)(OCC1OC(CC1O)N)O
InChI
1S/C15H31N3O13P2/c16-13-1-7(20)11(28-13)5-25-32(21,22)31-9-3-15(18)29-12(9)6-26-33(23,24)30-8-2-14(17)27-10(8)4-19/h7-15,19-20H,1-6,16-18H2,(H,21,22)(H,23,24)
InChI key
AWBASQCACWFTGD-UHFFFAOYSA-N
General description
Human placental DNA is isolated from a single female donor placenta, but will contain some maternal DNA. The sex has been determined using hybridization with DNA probes.
Application
Deoxyribonucleic acid from human placenta has been used:
- to compare the efficiency of DNA quantification methods
- as a standard in real-time PCR analysis of DNA samples from bladder cancer cell lines
- as an internal control to estimate the degree of fragmentation of circulating DNA
- for restriction digest analysis to identify low copy repeat regions of human chromosome 22q11
- to compare the 70-bp P5 exon sequence between DNA of different human and primate species
- to calculate copy number of genes, relative to normal human tissue
- in PCR reactions
For use in Southern hybridizations.
Other Notes
Solutions of DNA have been stored successfully for several months at 4 C, in 10 mM Tris, pH 7.5 - 8.0, with 1 mM EDTA and without a bacteriostatic agent. At low concentrations, about 25 μg/ml, DNA tends to absorb onto the surfaces of plastic tubes.
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Dieser Artikel | |||
|---|---|---|---|
| description buffered aqueous solution, sexed, female | description pUC18, buffered aqueous solution | description solution | description For hybridization |
| biological source human placenta | biological source Escherichia coli (RRI) | biological source - | biological source fish testis |
| grade Molecular Biology | grade Molecular Biology | grade Molecular Biology | grade Molecular Biology |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form buffered aqueous solution | form buffered aqueous solution | form solution | form solution |
| shipped in dry ice | shipped in dry ice | shipped in wet ice | shipped in dry ice |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
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Lagerklasse
11 - Combustible Solids
Was ist los?
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
PSA (Persönliche Schutzausrüstung)
Eyeshields, Gloves, type N95 (US)
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Hello I would like to know if this product contains RNA and if so, do you know the approximate amount of RNA/DNA ? Thank you in advance
1 answer-
Helpful?
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Hi, Could you please clarify what is the concentration (and the volume) of the D3035 product. Thank you
1 answer-
The concentration will be listed on the lot specific Certificate of Analysis. Please navigate to the ‘DOCUMENTATION’ section of the Product Detail Page to access a Certificate:
https://www.sigmaaldrich.com/product/sigma/d3035#product-documentationThe volume will depend on the pack size ordered and the concentration.
For instance, a 0.5 mg pack at 0.05 mg/ml will have a volume of at least 10ml.Helpful?
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