D5567

Anti-dimethyl-Histone H3 (diMe-Lys⁹) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Synonym(e): Anti-H3K9me2
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD06798437
• Conjugate: unconjugated
• Clone: polyclonal
• Application: ARR, ChIP, WB
• Citations: 18

Eigenschaften

• biological source: rabbit
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 17 kDa
• species reactivity: Drosophila, bovine, chicken, Arabidopsis thaliana, Caenorhabditis elegans, human, mouse, frog, rat
• technique(s): ChIP: suitable, microarray: suitable, western blot: 1:1,000-1:2,000 using whole extract of human epitheloid carcinoma HeLa cell line
• UniProt accession no.: P68431
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: dimethylation (Lys9)
• Gene Information: human ... H3F3A(3020), H3F3B(3021), HIST1H3A(8350), HIST1H3B(8358), HIST1H3C(8352), HIST1H3D(8351), HIST1H3E(8353), HIST1H3F(8968), HIST1H3G(8355), HIST1H3H(8357), HIST1H3I(8354), HIST1H3J(8356), HIST2H3A(333932), HIST2H3C(126961), HIST3H3(8290) ; mouse ... H3c10(319152), H3c11(319153), H3f3a(15078), H3f3b(15081), Hist1h3a(360198), Hist1h3b(319150), Hist1h3c(319148), Hist1h3d(319149), Hist1h3e(319151), Hist1h3f(260423), Hist1h3g(97908), Hist2h3b(319154), Hist2h3c1(15077), Hist2h3c2(97114) ; rat ... H3f3b(117056)

Beschreibung

General description

Histone methylation is a complex, dynamic process involved in a number of processes, including transcriptional regulation, chromatin condensation, mitosis and heterochromatin assembly. Conserved lysine residues in the N-terminal tail domains of histone H3, Lys4, Lys9 and Lys27 are the preferred sites of methylation. SET domain-, site-specific histone methyltransferases (HMTases) are involved in methylation of Lys9 in histone 3.

Immunogen

synthetic methylated peptide corresponding to amino acids 5-13 (diMe-Lys⁹) of human histone H3, conjugated to KLH. This sequence is identical in many species including mouse, rat, bovine, chicken, frog, Drosophila, C. elegans, tetrahymena, and Arabidopsis thaliana histone H3.

Application

Anti-dimethyl-Histone H3 (diMe-Lys⁹) antibody produced in rabbit has been used in immunoblotting and chromatin immunoprecipitation assay.

Biochem/physiol Actions

ChIP validated

Mono- and dimethylation of H3 at Lys9 are intrinsically linked to epigenetic silencing and heterochromatin assembly. Methylation of H3 at Lys9 generates a binding site for HP1 proteins, a family of heterochromatic adaptor proteins implicated in both gene silencing and in the organization of higher order chromatin.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable