This product has an osmolarity range of 275–304 mOsm/kg.
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D8537
Dulbeccos Phosphatgepufferte Kochsalzlösung
liquid, Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture
Dulbecco’s Phosphate Buffered Saline
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Synonym(e):
DPBS
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| Packungsgröße | SKU | Verfügbarkeit | Preis |
|---|---|---|---|
| 100 mL | Ab HEUTE lieferbarvonSchnelldorf | € 9,00 | |
| 500 mL | Ab HEUTE lieferbarvonSchnelldorf | € 22,40 | |
| 1 L | Ab HEUTE lieferbarvonSchnelldorf | € 40,30 | |
| 6 x 500 mL | Ab HEUTE lieferbarvonSchnelldorf | € 117,00 | |
| 6 x 1 L | Ab HEUTE lieferbarvonSchnelldorf | € 218,00 | |
| 24 x 500 mL | Ab HEUTE lieferbarvonSchnelldorf | € 421,00 |
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Technischer Dienst
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Unterstützung erhaltenProduktname
Dulbeccos Phosphatgepufferte Kochsalzlösung, Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture
Quality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
glucose: no (Dextro)
sodium pyruvate: no
shipped in
ambient
storage temp.
room temp
General description
Dulbeccos Phosphatgepufferte Kochsalzlösung (DPBS) ist eine ausgeglichene Salzlösung (BSS), die für die Handhabung und Kultivierung von Säugetierzellen eingesetzt wird. DPBS-Puffer wird zum Spülen, Waschen und Verdünnen von Säugetierzellen und Beibehaltung des osmotischen Gleichgewichts verwendet. Es stellt Wasser und anorganische Ionen bereit, die für den normalen Zellstoffwechsel unerlässlich sind. DPBS mit Calcium und Magnesium erleichtert die Bindung und Aggregation von Zellen. Dulbeccos PBS ohne diese Ionen kann zum Waschen und Spülen suspendierter Zellen eingesetzt werden. Der Phosphatpuffer hält den pH-Wert im physiologischen Bereich.
Application
Anwendungsgebiete von Dulbeccos Phosphatgepufferter Kochsalzlösung:
- Zum Verdünnen frischer Erythrozyten zur Untersuchung des Einfangens von Erythrozyten-Aggregaten.
- Zum Waschen der Wells einer Mikrotiterplatte.[1]
- Zum Waschen der Zellen vor dem Resuspendieren in Lysepuffer für die DNA-Isolierung.[2]
- Als Bestandteil des Embryo-Aufbewahrungsmediums zur Vitrifizierung von Embryonen.
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| Quality Level 300 | Quality Level 400 | Quality Level 400 | Quality Level 500 |
| form liquid | form liquid | form liquid | form liquid |
| storage temp. room temp | storage temp. - | storage temp. - | storage temp. room temp |
| impurities endotoxin, tested | impurities - | impurities - | impurities - |
| sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered |
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Lagerklasse
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| D8537-6X500ML | 04061838259400 |
| D8537-500ML | 04061838259387 |
| D8537-1L | 04061838259370 |
| D8537-6X1L | 04061838259394 |
| D8537-100ML | 04061833589724 |
| D8537-24X500ML | 04061833589731 |
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Eu gostaria de saber a osmolaridade da solução de DMPBS.
1 answer-
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Quería saber si éste producto se puede considerar RNAsa free para realizar durante la hibridación in situ
1 answer-
This product is a sterile-filtered M Quality Level 300 product. It is not molecular biology grade and does not have specifications such as RNase and DNase free. Therefore, this product cannot be considered RNase free for use in in situ hybridization.
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Bonjour, pourriez-vous m'indiquer le numéro de CAS de la solution de phosphate saline dulbecco sous forme liquide
1 answer-
This item is a multi-component product. A CAS number cannot be applied to this type of product. The Safety Data Sheet document includes the individual CAS numbers of those components that may be potentially hazardous.
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Hi, is this product GMP-grade or are GMP-garde material is used for the production of this?
1 answer-
This product is not GMP-grade. Unfortunately. a GMP-grade version of this product is not available.
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What is the concentration of the product in Molarity?
1 answer-
This is a multi-component formulation. A 'molarity' is not reported for this type of product. See below for the list of components:
Product D8537 contains:
Potassium chloride: 0.2 g/l
Potassium phosphate monobasic: 0.2 g/l
Sodium chloride: 8 g/l
Sodium Phosphate dibasic: 1.15 g/lPlease see the link below for additional information:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/124/464/d8537for.pdfHelpful?
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What is the bottle thread type on the 1L bottle (D8537-1L)
1 answer-
The product is packaged in a square PET bottle with an HDPE cap. The cap specifications are as follows:
Thread Type 38-430
Thread Diameter 37.3 mmHelpful?
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is a Salt Toxicity Test?
1 answer-
The Cell Culture assay is called a Salt Toxicity Test (previously called Cell Culture Salt Testing). A more complete description is below:Cells are harvested and counted. They are resuspended in DPBS and incubated at 37°C, 5% CO2 for 1 hour. The cells are counted and %viability determined. The cells are then cultured for an additional 7 hours (total of 8 hours). The cells are counted again and the % viability determined. The viability at 1 hr has to be >95% of the original viability. The viability at 8 hrs must be >75% of the original viability.
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What is the pH of a solution of Product D8537, Dulbecco's Phosphate?
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The pH will be in the range of 7.1-7.5, according to the specification sheet.
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Is Product D8537, Dulbecco's Phosphate, a 1X or 10 X concentration?
1 answer-
This is a 1X concentration. The 10X concentration is listed under product D1408.
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