This product reconstituted to 0.365 g/L yields a molarity of approximately 0.0025 mol/L (2.5 mM). Please see the link below to review the full formulation:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/822/486/d0547pis-ms.pdf
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D8900
Dulbecco Modifiziertes Eagle-Medium/Nährstoffmischung F-12 Ham
With L-glutamine and 15 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture
Synonym(e):
DME/F-12, 1:1 Mischung
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| Größe/SKU | Verfügbarkeit | Preis |
|---|---|---|
10 x 1 L | Warenkorb auf Verfügbarkeit prüfen | € 111,00 |
10 L | Warenkorb auf Verfügbarkeit prüfen | € 111,00 |
50 L | Warenkorb auf Verfügbarkeit prüfen | € 426,00 |
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Technischer Dienst
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Unterstützung erhaltenQuality Segment
form
powder
technique(s)
cell culture | mammalian: suitable
components
sodium pyruvate: 0.055 g/L
HEPES: 15 mM
NaHCO3: no
L-glutamine: yes
glucose: 3.15 g/L
phenol red: yes
shipped in
ambient
storage temp.
2-8°C
General description
Application
Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham is suitable for the growth of a wide range of cell types in low serum conditions. It has been used:
- as a component of the culture medium for endometrial explants in vitro culture
- to immerse chitosan-gelatin-glycerol phosphate hydrogels for cytotoxicity evaluation
- to culture nucleus pulposus (NP) cells to culture human articular chondrocytes for evaluating the toxicity of baicalein
Preparation Note
Formulated to contain 15.6 grams of powder per liter of medium.
Supplement with 1.2 g/L sodium bicarbonate.
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Dieser Artikel | |||
|---|---|---|---|
| description With L-glutamine and 15 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture | description With L-glutamine and 15 mM HEPES, without sodium bicarbonate and phenol red, powder, suitable for cell culture | description With L-glutamine and sodium bicarbonate, without HEPES, liquid, sterile-filtered, suitable for cell culture | description With 15 mM HEPES, without L-glutamine, L-leucine, L-lysine, L-methionine, CaCl2, MgCl2, MgSO4, sodium bicarbonate, and phenol red, powder, suitable for cell culture |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| form powder | form powder | form liquid | form powder |
| components sodium pyruvate: 0.055 g/L | components NaHCO3: no | components glucose: 3.15 g/L | components L-glutamine: no |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
| Quality Level 500 | Quality Level 500 | Quality Level 400 | Quality Level - |
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Lagerklasse
11 - Combustible Solids
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WGK 1
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What is the molarity of L-glutamine when reconstituted?
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What is the molarity of glucose when reconstituted with water?
1 answer-
Upon reconstitution, the molarity of glucose is 17.5 mM (3.15 g/L).
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