E4274

Endotoxin Removal solution

Remove lipopolysaccharides (LPS) from plasmid DNA preparations

• Synonym(e): ET removal buffer, ET removal reagent, ET removal solution, endo removal buffer, endo removal reagent, endo removal solution, endotoxin contamination removal, endotoxin removal buffer, endotoxin removal reagent, plasmid purification endo, plasmid purification endo free
• NACRES: NA.52
• UNSPSC Code: 12352200

Eigenschaften

• Quality Segment: 200

Beschreibung

Application

Endotoxin Removal solution has been used to remove lipopolysaccharide (LPS) from Baker′s yeast RNA.

Biochem/physiol Actions

The reagent relies on the micellar properties of Triton X-114 to remove endotoxins from solution. When warmed above 20 °C, micelles aggregate into a separate phase that sequesters amphiphilic solutes, including lipopolysaccharides. The hydrophilic DNA remains in the aqueous phase.

Preparation Note

1 volume treats 5 volumes of DNA solution (≤1mg/ml)

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable