The total unit of E5546-50UG is not provided as the product is characterized in micrograms (ug).
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E5546
Erythropoietin (EPO) human
recombinant, expressed in HEK 293 cells, suitable for cell culture
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Synonym(e):
EPO, epoetin, hematopoietin, hemopoietin
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| Größe/SKU | Verfügbarkeit | Preis |
|---|---|---|
50 μg | Warenkorb auf Verfügbarkeit prüfen | € 253,00 |
Über diesen Artikel
NACRES:
NA.77
UNSPSC Code:
12352202
Form:
lyophilized powder
Assay:
≥98% (SDS-PAGE)
Recombinant:
expressed in HEK 293 cells
Technischer Dienst
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Unterstützung erhaltenrecombinant
expressed in HEK 293 cells
Quality Segment
assay
≥98% (SDS-PAGE)
form
lyophilized powder
potency
≤2.5 ng/mL ED50 (measured in a cell proliferation assay using TF-1 human cells)
technique(s)
cell culture | mammalian: suitable
impurities
Endotoxin, tested
storage temp.
−20°C
General description
Recombinant human erythropoietin (EPO) is expressed in human HEK 293 cells as a glycoprotein with a calculated molecular mass of ~18 kDa. This protein is manufactured in human cells using an all-human production system, with full chemically defined ingredients and with no serum. The human cells expression system allows human-like glycosylation and folding, and often supports better stability of the protein in culture. The protein is produced with no artificial tags.
Biochem/physiol Actions
Erythropoietin (EPO) is a glycoprotein hormone, known for its erythropoietic activity. It is used as treatment for anemia in humans with renal failure or cancer. EPO also reduces the amount of blood transfusions required on premature infants and surgeries.
EPO consists of 165 amino acids and has a high carbohydrate content, of both N-linked and O-linked types. The oligosaccharide chains are responsible for EPO production, secretion, longevity, and functioning. The biological activity of EPO depends upon two disulfide bonds, one formed between cysteine7 and cysteine160, and the other between cysteine29 and cysteine33. Thus, EPO proper folding, glycosylation and disulfide bonds formation are crucial for its function.
EPO was found to downregulate HIF-1α in the diabetic rat model. EPO helped maintaining the homeostasis of intracellular zinc in retinal cells, altered as a result of diabetes, by restoring Zinc transporter 8 (ZnT8) expression.
EPO consists of 165 amino acids and has a high carbohydrate content, of both N-linked and O-linked types. The oligosaccharide chains are responsible for EPO production, secretion, longevity, and functioning. The biological activity of EPO depends upon two disulfide bonds, one formed between cysteine7 and cysteine160, and the other between cysteine29 and cysteine33. Thus, EPO proper folding, glycosylation and disulfide bonds formation are crucial for its function.
EPO was found to downregulate HIF-1α in the diabetic rat model. EPO helped maintaining the homeostasis of intracellular zinc in retinal cells, altered as a result of diabetes, by restoring Zinc transporter 8 (ZnT8) expression.
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Dieser Artikel | |||
|---|---|---|---|
| description recombinant, expressed in HEK 293 cells, suitable for cell culture | description recombinant, expressed in insect cells, suitable for cell culture | description recombinant, expressed in NSO cells, ≥90% (SDS-PAGE), lyophilized powder, suitable for cell culture | description >90% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder |
| assay ≥98% (SDS-PAGE) | assay - | assay ≥90% (SDS-PAGE) | assay >90% (SDS-PAGE) |
| recombinant expressed in HEK 293 cells | recombinant expressed in insect cells | recombinant expressed in NSO cells | recombinant expressed in NSO cells |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) - |
| form lyophilized powder | form - | form lyophilized powder | form lyophilized powder |
| impurities Endotoxin, tested | impurities endotoxin, tested | impurities endotoxin, tested | impurities endotoxin, tested |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
Lagerklasse
11 - Combustible Solids
Was ist los?
WGK 2
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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