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Merck

F6136

Anti-Horse Spleen Ferritin antibody produced in rabbit

affinity isolated antibody, lyophilized powder

Synonym(e):

Ferritin Antibody

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1 MG

€ 431,00

€ 431,00


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MDL-Nummer:
UNSPSC-Code:
12352203
NACRES:
NA.46

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Unterstützung erhalten

Biologische Quelle

rabbit

Konjugat

unconjugated

Antikörperform

affinity isolated antibody

Antikörper-Produkttyp

primary antibodies

Klon

polyclonal

Form

lyophilized powder

Speziesreaktivität

horse

Methode(n)

immunoelectrophoresis: suitable
immunoelectrophoresis: suitable
indirect ELISA: 1:10,000

Lagertemp.

2-8°C

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

horse ... Ftl(100051593)

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Dieser Artikel
F5012H8890MABC602
antibody form

affinity isolated antibody

antibody form

whole antiserum

antibody form

whole antiserum

antibody form

purified immunoglobulin

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

mouse

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

-

form

lyophilized powder

form

liquid

form

-

form

-

technique(s)

immunoelectrophoresis: suitable, indirect ELISA: 1:10,000

technique(s)

Ouchterlony double diffusion: 1:16

technique(s)

immunoelectrophoresis: suitable

technique(s)

immunohistochemistry: suitable, western blot: suitable

Allgemeine Beschreibung

Ferritin consists of homogenous protein segment called as apoprotein and an iron segment which binds to the apoprotein. It is composed of heavy (H) and light (L) chains.
Ferritin is the storage form of iron in mammals, found mainly in liver, bone marrow and spleen. It is also present in small intestine, placenta, kidney and heart. Horse spleen ferritin is made up of 24 identical subunits. Ferritin of different organs is distinct with different electrophoretic mobility and possible functional differences.
.

Immunogen

Purified horse spleen ferritin

Anwendung

Anti-Horse Spleen Ferritin antibody was used for immunocytochemistry of rat brain sections at a working antibody dilution of 1:2000 and in mouse brain sections at 1:1000. The antibody was used for immunoblotting using cell lysates of mouse primary erythroblasts and human ovarian carcinoma cells and human bronchial epithelial cells at a working dilution of 1:1000. The antibody is suitable for immunoelectrophoresis.
Horse Spleen Ferritin antibody has been used:
  • immunofluorescence
  • in western blotting
  • in immunohistochemistry

Biochem./physiol. Wirkung

Ferritin iron is oxidized from Fe(II) to Fe(III) in the presence of the enzyme ferroxidase.
The antibody specifically recognizes ferritin from horse spleen.

Physikalische Form

Lyophilized from 0.01 M sodium phosphate, 0.015 M sodium chloride, pH 7.2

Angaben zur Herstellung

Reconstitute with 0.135 M sodium chloride.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)


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Die Dokumentenbibliothek aufrufen

R R Crichton et al.
The Biochemical journal, 131(1), 51-59 (1973-01-01)
1. Ferritin was isolated from human and horse spleen and liver, and apoferritin prepared therefrom. 2. The electrophoretic mobilities of the four apoferritins were determined on polyacrylamide gels and on cellulose acetate strips, and all found to be equal. 3.
Structure and physiological functions of ferritin
Granick, S
Physiological Reviews, 31(4), 489-511 (1951)
Jennifer R Charlton et al.
Nanomedicine : nanotechnology, biology, and medicine, 12(6), 1735-1745 (2016-04-14)
Ferritin is a naturally occurring iron storage protein, proposed as a clinically relevant nanoparticle with applications as a diagnostic and therapeutic agent. Cationic ferritin is a targeted, injectable contrast agent to measure kidney microstructure with MRI. Here, the toxicity of
Matthias Schranzhofer et al.
Blood, 107(10), 4159-4167 (2006-01-21)
Terminal erythropoiesis is accompanied by extreme demand for iron to ensure proper hemoglobinization. Thus, erythroblasts must modify the "standard" post-transcriptional feedback regulation, balancing expression of ferritin (Fer; iron storage) versus transferrin receptor (TfR1; iron uptake) via specific mRNA binding of
Microglial pathology in Down syndrome
Xue Q S and Streit W J
Acta Neuropathologica, 122(4), 455-455 (2011)

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