F9925

Anti-FLIPγ/δ, C-Terminal antibody produced in rabbit

1 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(e): Anti-CASH, Anti-CLARP, Anti-FLAME-1, Anti-I-FLICE
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD02096351
• Conjugate: unconjugated
• Clone: polyclonal
• Application: ICC, IF, WB
• Citations: 10

Eigenschaften

• biological source: rabbit
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 25 kDa (FLIPδ/FLIP_S), antigen 35 kDa (FLIPγ)
• species reactivity: human, mouse
• concentration: 1 mg/mL
• technique(s): immunocytochemistry: 2 μg/mL, immunofluorescence: 10 μg/mL, western blot: 1:1,000
• UniProt accession no.: O15519
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... CFLAR(8837) ; mouse ... Cflar(12633)

Beschreibung

General description

In human Viral FLICE-inhibitory proteins (v-FLIPs) is identified as c-FLIP. It is composed of two death effector domains which have structural resemblance with the N-terminal half of caspase-8 and a caspase-like domain. It exist as multiple splice variants: FLIP α, β, γ and δ. Along with splice variants, it has two endogenous forms of the protein − c-FLIP_long and c-FLIP_short.

Immunogen

FLIP antibody was raised against a 19 amino acid peptide near the carboxy terminus of human FLIP.
The immunogen is located within amino acids 180 - 230 of FLIP.

Application

Anti-FLIPγ/δ, C-Terminal antibody is suitable for microarray and western blot at a dilution of 1:1,000 using HeLa, Jurkat, THP-1, A431, K562, and NIH-3T3 cell lysates.

Biochem/physiol Actions

C-FLIP plays an important role in apoptosis signaling pathways. It acts as proapoptotic molecule or as an anti-apoptotic molecule. It has been reported that c-FLIP can interact with both FADD and caspase-8. It prevents caspase-8 recruitment and processing through DED-DED (Death Effector Domain) interaction followed by CD95-induced apoptosis.

Physical form

Solution in 0.01 M phosphate buffered saline containing 0.02% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids