G1163

O-Glycosidase from Streptococcus pneumoniae

recombinant, expressed in E. coli, buffered aqueous solution

• Synonym(e): Endo-α-N-acetylgalactosaminidase, O-Glycanase
• CAS-Nummer: 9032-92-2
• EG-Nummer: 3.2.1.97 (BRENDA, IUBMB)
• UNSPSC Code: 12352204
• EC Number: 232-888-2
• NACRES: NA.32
• MDL number: MFCD12922510
• Recombinant: expressed in E. coli
• Concentration: ≥800 units/mL

Eigenschaften

• recombinant: expressed in E. coli
• Quality Segment: 200
• conjugate: (O-linked)
• form: buffered aqueous solution
• mol wt: 180 kDa
• concentration: ≥800 units/mL
• shipped in: wet ice
• storage temp.: 2-8°C

Beschreibung

Biochem/physiol Actions

Releases unsubstituted Ser- and Thr-linked β-Gal-(1→3)-α-GalNAc (Core 1 type O-glycan) from glycoproteins. Substitutions of the disaccharide core with sialic acid, lactosamine (galactose-N-acetyl glucosamine), or fucose will block hydrolysis and prevent the liberation of the oligosaccharide from the protein. Pretreament with glycolytic enzymes to remove substituent saccharides from the O-glycan may be needed prior to cleavage using O-glycosidase..

Packaging

Supplied with 5× Reaction Buffer, 250 mM NaH₂PO₄ pH 5.0.

Physical form

Solution in 50 mM sodium phosphate, pH 7.5

Analysis Note

Screened for presence of: β-galactosidase, α-mannosidase, β-hexosaminidase, α-fucosidase, neuraminidase, and proteases. See Certificate of Analysis for lot specific information.

Other Notes

One unit will hydrolyze 1 μmole of p-nitrophenyl galacto-N-bioside (β-Gal-(1→3)-α-GalNAc-1→ΟC₆H₄NO₂) per min at 37 °C at pH 6.5.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• PSA (Persönliche Schutzausrüstung): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)