G9785

GSEM-Zusatz

liquid, suitable for cell culture,

• Synonym(e): Glutaminsynthetase-Expressionsmedienzusatz
• MDL number: MFCD00677096
• NACRES: NA.75
• UNSPSC Code: 12352205

Eigenschaften

• Produktname: GSEM-Zusatz, 50 ×, liquid, sterile-filtered, suitable for cell culture
• sterility: sterile-filtered
• Quality Segment: 200
• form: liquid
• concentration: 50 ×
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• shipped in: dry ice
• storage temp.: −20°C

Beschreibung

General description

Glutamine synthetase expression medium (GSEM) supplement enables formation of glutamine from ammonia and glutamate there by alleviating accumulation of ammonia in culture medium. It is exploited for high yield recombinant protein production in Chinese hamster ovary (CHO) cells.

Application

GSEM Supplement has been used as a supplement for ProCHO5 Protein-free medium for culturing Chinese hamster ovary (CHO) cells and selective medium for NS0 myeloma cells.

Packaging

Contains (mg/L): L-alanine 450.0, L-asparagine · H₂O 4261.0, L-aspartic acid 650.0, L-glutamic acid 3750.0, L-proline 575.0, L-serine 500.0, adenosine 350.0, guanosine 350.0, cytidine 350.0, uridine 350.0, and thymidine 12.0

Preparation Note

One mL will supplement 50 mL of cell culture medium.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable