GEE70073X

Exonuclease I, 10 U/μL

Cytiva E70073X, pack of 5000 units

• Synonym(e): Exonuclease I Enzyme, ExoI, PCR cleanup enzyme, single-stranded DNA exonuclease
• NACRES: NA.53
• UNSPSC Code: 12352204
• Biological source: Escherichia coli

Eigenschaften

• biological source: Escherichia coli
• packaging: pack of 5000 units
• manufacturer/tradename: Cytiva E70073X
• shipped in: dry ice
• storage temp.: −20°C

Beschreibung

General description

Acts specifically on single-stranded DNA degrading it processively in the 3′- to 5′-direction, producing 5′-mononucleotides.

Exonuclease I (Exo I) from E. coli is an Mg²⁺ ion dependent exonuclease.

Application

Exonuclease I has been used to quantify the expression of 56 genes in each F2 XY gonad.

Biochem/physiol Actions

Exonuclease I (Exo I) from E. coli can degrade single-stranded DNA (ssDNA) from 3′ to 5′. It participates in various DNA repair systems, like, RecBCD-dependent homologous recombination, methyl-dependent mismatch repair and genomic tandem repeat maintenance.

Features and Benefits

• Elimination of residual single-stranded DNA containing a 3′-terminus.
• Measuring endonucleolytic cleavage of covalently closed circular (ccc) ssDNA.
• Measuring DNA helicase activity.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids