I2276

Interleukin-12 human

≥97% (SDS-PAGE and N-terminal analysis), recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture

• Synonym(e): IL-12
• UNSPSC Code: 12352202
• NACRES: NA.32
• MDL number: MFCD00212975

Eigenschaften

• biological source: human
• Quality Segment: 200
• recombinant: expressed in baculovirus infected Sf21 cells
• assay: ≥97% (SDS-PAGE and N-terminal analysis)
• form: lyophilized powder
• potency: 0.01-0.05 ng/mL ED₅₀
• mol wt: glycoprotein 75 kDa
• packaging: pkg of 5 μg
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• UniProt accession no.: P29459,; P29460
• storage temp.: −20°C
• Gene Information: human ... IL12A(3592), IL12B(3593)

Beschreibung

General description

Recombinant human Interleukin‑12 (IL‑12) is expressed in baculovirus‑infected Sf21 insect cells and supplied as a lyophilized glycoprotein. The biological activity of recombinant human IL‑12 was measured by its ability to stimulate the proliferation of PHA‑activated human T lymphoblasts. IL‑12 has anti‑angiogenic properties.

Application

Recombinant human IL‑12 has been used in mammalian cell culture research applications, including studies of T‑cell and natural killer (NK) cell activation and cytokine‑mediated signaling pathways in vitro.

Biochem/physiol Actions

Interleukin‑12 (IL‑12) is an immunomodulatory cytokine that mediates cellular responses through activation of T cells and natural killer (NK) cells to produce IFN‑γ. Recombinant human IL‑12 has been widely studied in mammalian cell culture models investigating immune‑related signaling and cytokine‑driven cellular activation pathways.

Interleukin-12 (IL-12) was identified as a factor secreted by human Epstein-Barr (EBV)-transformed B cell lines. IL-12, or Natural Killer Cell Stimulatory Factor (NKSF), is a 75 kDa disulfide-linked heterodimer of a 35 kDa subunit and 40 kDa subunit. IL-12 is produced predominantly by monocytes and NK cells and induces T cells and NK cells to produce IFN-γ. Human IL-12 is not active on mouse cells, but murine IL-12 is active on both murine and human lymphocytes. The biological activity of recombinant human IL-12 was measured by its ability to stimulate the proliferation of PHA-activated human T lymphoblasts. IL-12 has anti-angiogenic properties.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.25 mg bovine serum albumin.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• PSA (Persönliche Schutzausrüstung): Eyeshields, Gloves, type N95 (US)