I9646

IL-6 mouse

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonym(e): Interleukin-6, mIL-6, IL-6
• UNSPSC Code: 12352202
• NACRES: NA.77
• MDL number: MFCD01656039
• eCl@ss: 32160701
• Biological source: mouse
• Recombinant: expressed in E. coli
• Assay: ≥97% (SDS-PAGE)
• Form: lyophilized powder
• Mol wt: ~21.7 kDa
• Impurities: ≤1.000 EU/mg Endotoxin level

Eigenschaften

• Produktname: Interleukin-6 from mouse, IL-6, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture, carrier free
• biological source: mouse
• Quality Segment: 200
• recombinant: expressed in E. coli
• assay: ≥97% (SDS-PAGE)
• form: lyophilized powder
• potency: 0.02-0.2 ng/mL EC₅₀
• quality: endotoxin tested
• mol wt: ~21.7 kDa
• packaging: pkg of 5 and 25 μg
• technique(s): cell culture | mammalian: suitable
• impurities: ≤1.000 EU/mg Endotoxin level
• color: white
• UniProt accession no.: P08505
• storage temp.: −20°C

Beschreibung

General description

Recombinant mouse Interleukin‑6 (IL‑6) is a multifunctional cytokine expressed in E. coli and supplied as a carrier‑free lyophilized powder. IL‑6 is widely used in mammalian cell culture research involving immune and inflammatory signaling pathways in vitro.

Application

Recombinant mouse IL‑6 has been used in mammalian cell culture research applications, including immune cell proliferation assays and cytokine‑mediated signaling studies in vitro.

Biochem/physiol Actions

Interleukin‑6 (IL‑6) is an immunomodulatory cytokine that supports proliferation and differentiation of multiple cell types. It acts upon a variety of cells, including fibroblasts, myeloid progenitor cells, T cells, B cells and hepatocytes. IL-6 is involved in the responses that occur after infection and injury and may prove to be as important as IL-1 and TNF-α in regulating the acute phase response.

Interleukin-6 (IL-6) is a multifunctional protein originally discovered in the media of cells stimulated with double stranded RNA. IL-6 appears to be directly involved in the responses that occur after infection and injury and may prove to be as important as IL-1 and TNF-α in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages, and endothelial cells. It acts upon a variety of cells, including fibroblasts, myeloid progenitor cells, T cells, B cells and hepatocytes. IL-6 induces multiple effects, as indicated by its numerous synonyms: plasmacytoma growth factor (PCT-GF), interferon-β-2 (IFN-β₂), monocyte derived human B cell growth factor, B cell stimulating factor (BSF-2), hepatocyte stimulating factor (HSF), Interleukin Hybridoma/Plasmacytoma-1 (IL-HP1). In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 also potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors.

Physical form

Lyophilized from a sterile filtered solution with no additives.

Analysis Note

The biological activity of recombinant mouse IL-6 was measured in a cell proliferation assay using T1165.85.2.1 cells.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• PSA (Persönliche Schutzausrüstung): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)