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K3753

Krebs-Henseleit Buffer Modified

With 2000 mg/L glucose, without calcium chloride and sodium bicarbonate, powder, suitable for cell culture

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UNSPSC Code:
12352207
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Quality Segment

form

powder

technique(s)

cell culture | mammalian: suitable

storage temp.

2-8°C

General description

Krebs-Henseleit Buffer Modified is a balanced salt solution (BSS) that is used in cell culture and plays a role in providing cells with water and inorganic salts for normal cell metabolism. In addition, it provides a buffering system to maintain the optimal pH of the medium and acts as an irrigating, transporting, and diluting agent to maintain the intra- and extra-cellular osmotic balance. BSS provides glucose as an energy source for cell metabolism.

Application

Krebs-Henseleit Buffer Modified has been used:

  • to collect the rat brains for ex vivo brain slice experiments
  • to measure calcium and sodium in rat pulmonary arterial endothelial cells (PAECs)
  • to collect the uterine and forelimb blood vessel samples from horses

Preparation Note

Formulated to contain 9.6 grams of powder per liter of medium.
Supplement with 2.1 g/L sodium bicarbonate.

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Dieser Artikel
K4002P85741805
Sigma-Aldrich

Sigma-Aldrich

K3753

Krebs-Henseleit Buffer Modified

Sigma-Aldrich

Sigma-Aldrich

K4002

Krebs-Ringer Bicarbonate Buffer

Natriumpyruvat BioXtra, ≥99%

Sigma-Aldrich

P8574

Natriumpyruvat

Phosphate Buffer powder

Sigma-Aldrich

1805

Phosphate Buffer

description

With 2000 mg/L glucose, without calcium chloride and sodium bicarbonate, powder, suitable for cell culture

description

With 1800 mg/L glucose, without calcium chloride and sodium bicarbonate, powder, suitable for cell culture

description

BioXtra, ≥99%

description

powder

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

-

Quality Level

500

Quality Level

500

Quality Level

200

Quality Level

500

form

powder

form

powder

form

powder

form

powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

room temp

Lagerklasse

11 - Combustible Solids

Was ist los?

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

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Questions

  1. How long can Krebs solution be stored after it is being reconstituted?

    1 answer

    1. Stability data for Krebs-Henseleit Buffer Modified (cat# K3753) when reconstituted is not available. Stability will also depend on the supplements added to the solution.

      It is generally accepted that the solution can be stored between 2 and 8°C for several months, on the condition that it has been filtered using a 0.2µm membrane in order to make sterile. If it is not sterile, it must be used within 24 hours.

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