As listed in the 'DESCRIPTION' section of the product page, under 'Other Notes', this product contains non-animal derived fatty acids (2 μg/ml arachidonic and 10 μg/ml each linoleic, linolenic, myristic, oleic, palmitic and stearic), 0.22 mg/ml cholesterol from New Zealand sheep's wool, 2.2 mg/ml Tween-80, 70 μg/ml tocopherol acetate and 100 mg/ml Pluronic F-68 solubilized in cell culture water.
L0288
Lipid Mixture 1, Chemically Defined
liquid, sterile-filtered, BioReagent, suitable for cell culture
Synonym(e):
lipid mixture
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100 ML
€ 99,90
Über diesen Artikel
UNSPSC-Code:
12352211
NACRES:
NA.75
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Unterstützung erhaltenSterilität
sterile-filtered
Produktlinie
BioReagent
Form
liquid
Methode(n)
cell culture | mammalian: suitable
Verunreinigungen
≤10 EU/mL endotoxin
Lagertemp.
2-8°C
Verwandte Kategorien
1 of 4
Dieser Artikel | L5146 | D0547 | D0697 |
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | insect: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| form liquid | form liquid | form powder | form liquid |
| Quality Level 500 | Quality Level 500 | Quality Level 500 | Quality Level 400 |
| sterility sterile-filtered | sterility sterile-filtered | sterility - | sterility sterile-filtered |
| product line BioReagent | product line BioReagent | product line - | product line - |
Allgemeine Beschreibung
Lipid mixture 1, Chemically defined is recommended for use in cell culture at 1 to 10 mL per liter of medium.
Anwendung
Lipid Mixture 1, Chemically Defined has been used:
- as a component of bovine serum albumin (BSA) containing Bracket-Oliphant (BO) medium for the resuspension of sperm pellet[1]
- as a supplement in spheroid media for culturing primary human subcutaneous stromal vascular fraction cells[2]
- to prepare a cell steatosis model for culturing of mouse and human hepatocytes[3]
Sonstige Hinweise
Contains non-animal derived fatty acids (2 μg/ml arachidonic and 10 μg/ml each linoleic, linolenic, myristic, oleic, palmitic and stearic), 0.22 mg/ml cholesterol from New Zealand sheep′s wool, 2.2 mg/ml Tween-80, 70 μg/ml tocopherol acetate and 100 mg/ml Pluronic F-68 solubilized in cell culture water.
The final product contains 10% ethanol by volume.
The final product contains 10% ethanol by volume.
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Produkt-Nr.
Beschreibung
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Lagerklassenschlüssel
10 - Combustible liquids
WGK
WGK 1
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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The anti-inflammatory, immunomodulatory, and anticancer effects of micheliolide (MCL) isolated from Michelia champaca were previously reported, but its role and underlying mechanisms in relieving liver steatosis remain unclear. Herein, we investigated the effects of MCL on hepatic steatosis using a
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Saturated fatty acids accelerate linear motility through mitochondrial ATP production in bull sperm.
Md Mazharul Islam et al.
Reproductive medicine and biology, 20(3), 289-298 (2021-07-16)
The present study was undertaken to clarify whether bovine sperm could take up fatty acids (FAs) and produce ATP to maintain linear motility. Frozen bovine semen was thawed in media containing either lipid mixture (LM) or FAs, and sperm motility
Semir Beyaz et al.
Nature, 531(7592), 53-58 (2016-03-05)
Little is known about how pro-obesity diets regulate tissue stem and progenitor cell function. Here we show that high-fat diet (HFD)-induced obesity augments the numbers and function of Lgr5(+) intestinal stem cells of the mammalian intestine. Mechanistically, a HFD induces
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How the unsaturated fatty acid, oleic acid and other cell culture components affect the performance of serum-free, protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies.
How palmitic acid, other fatty acids and other cell culture components affect the performance of serum-free, protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies.
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組成を教えて頂けませんでしょうか？
1 answer-
Helpful?
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Lipid Mixture 1, Chemically Defined(L0288) 제품 설명에서 non-animal source라고 되어 있는데 "0.22 mg/ml cholesterol from New Zealand sheep′s wool" 라는 문구가 있습니다. 이를 보면 콜레스테롤의 경우 양모에서 추출한 것 같은데, 혹시 이 성분은 animal source가 맞는건지 아니면 non-animal source인 건지 궁금합니다.
1 answer-
L0288 is not derived from animal origin except for 2003140 cholesterol as stated in the sample CoA on the product site.
Helpful?
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안녕하세요. 위 제품에는 BSA (Bovine serum albumin)이 구성요소에 포함되어 있는것일까요? 아니면 BSA구성요소의 일부가 포함되어 있는 것일까요? BSA대체제로 사용할 수 있는 것일까요?
1 answer-
As listed in the DESCRIPTION section, this product has been cited as a component of bovine serum albumin in a Bracket-Oliphant (BO) medium for the resuspension of sperm pellets. However, this product is typically used as a medium supplement in mammalian cell culture. While there are fatty acids present in BSA, this product alone is not a suitable replacement for bovine serum albumin. Other supplements would be required. The supplement profile would depend on the type of cell being cultured.
To discuss further, please navigate to the link https://www.sigmaaldrich.com/techservice, click on "Product Technical Inquiries" under the Products Section, and respond with all the required information so that a member of the Technical Service team can reach out to assist further.
For additional information, please see the link below to review the product datasheet for this supplement product as well as others:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/309/806/i3146pis.pdfHelpful?
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