We do not test for the coefficient of curvature of product L4655. However, the mean diameter of these beads are 0.90 - 1.10 micron.
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L4655
Latex-Beads, carboxylatmodifiziertes Polystyrol, gelb-grün fluoreszierend
aqueous suspension, 1.0 μm mean particle size
Synonym(e):
Fluoreszierende Latex-Beads
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| Größe/SKU | Verfügbarkeit | Preis |
|---|---|---|
1 mL | Warenkorb auf Verfügbarkeit prüfen | € 112,00 |
Über diesen Artikel
Technischer Dienst
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Unterstützung erhaltenform
aqueous suspension
Quality Segment
composition
Solids, 2.5%
technique(s)
cell based assay: suitable
mean particle size
1.0 μm
fluorescence
λex ~470 nm; λem ~505 nm
suitability
suitable for immunoassay
application(s)
cell analysis
Application
Latex-Beads, Carboxylat-modifiziertes Polystyrol, mit gelb-grüner Fluoreszenz haben folgende Anwendungen:
- bei der Herstellung von Konjugaten mit dem epidermalen Wachstumsfaktor für Studien mit Bildgebung mittels indirekter Immunfluoreszenz
- zum Kultivieren von bronchoalveolären Makrophagen sowie von Makrophagen, die aus menschlichen Monozyten gewonnen wurden, vor Bildgebungsstudien zur Phagozytose
- gemeinsam mit von Salmonella exprimiertem, grün fluoreszierendem Protein (GFP) für konfokale mikroskopische Untersuchungen und in der Biofluoreszenz-Bildgebung
Carboxylat-modifizierte Polystyrol-Latex-Beads wurden genutzt, um morphofunktionale Daten über das Immunsystem von Carabus lefebvrei zu sammeln, einem in Italien endemischen Laufkäfer, der sich von Schnecken ernährt und oft als Indikator für die Lebensraumqualität der Bergwälder im Apennin dient. Carboxylat-modifizierte Polystyrol-Latex-Beads wurden außerdem in Vergleichsstudien eingesetzt, um Informationen über die adulten Käfer und Larven von Cetonischema aeruginosa zu gewinnen und die phagozytische Aktivität von kultivierten Schilddrüsenzellen der Ratte zu untersuchen.
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Dieser Artikel | |
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| description aqueous suspension, 1.0 μm mean particle size | description aqueous suspension, 1.0 μm mean particle size |
| suitability suitable for immunoassay | suitability - |
| technique(s) cell based assay: suitable | technique(s) cell based assay: suitable |
| fluorescence λex ~470 nm; λem ~505 nm | fluorescence λex ~470 nm; λem ~505 nm |
| form aqueous suspension | form aqueous suspension |
| application(s) cell analysis | application(s) cell analysis |
| Quality Level 100 | Quality Level 100 |
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Lagerklasse
10 - Combustible liquids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
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What is the coefficient of curvature of the particle?
1 answer-
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What is the refractive index of the particle?
1 answer-
The refractive index value for polystyrene is 1.591 at 590 nm.
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What is the concentration of product L4655?
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The concentration of the Latex Beads is approximately 4.75x10^10 beads/mL.
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Could you tell me the particles/ml of the L4655 product? If it's 2.5%, can I say it’s 25 mg/ml?
1 answer-
This suspension contains 2.5 g/100 mL or 25 mg/mL solids. The calculated particle count is approximately 4.75 x 10E10 beads per mL.
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What are the ingredients of the solution in which the plastic is diluted? Can I dilute it in PBS?
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This product is prepared in water containing a prioprietary anionic surfactant and >0.1% Sodium Azide. Phosphate Buffered Saline is a suitable diluent.
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What are the differences between carboxylate-modified polystyrene and amine-modified polystyrene Latex beads, and which type of beads is suitable for phagocytosis applications?
1 answer-
When selecting beads for phagocytosis assays, there are three key parameters to consider: the size of the bead, the color of the bead (dyed or undyed), and the modification of the bead (carboxylate, amine, or sulfate modified).
Beads chosen for phagocytosis experiments typically range from approximately 0.5 to 3 micrometers in size. A 1:250 dilution of the beads into the culture medium is commonly used for phagocytosis experiments. Latex Beads, 2 um (Product No. L0905), can be utilized for phagocytosis assays, as macrophages can phagocytose particles larger than 2 um.
Fluorescent latex beads are available with different fluorescent dyes incorporated into the beads. The yellow-green beads are commonly used for phagocytosis studies and are compatible with most flow cytometers and fluorescent microscopes.
For phagocytosis applications, the choice of bead modification (carboxylate vs. amine) depends on the specific assay being conducted. Carboxylate-modified beads, with a negative charge, mimic the uptake of apoptotic cells, while amine-modified beads, with a positive charge, are used to mimic the uptake of opsonized cells.
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Are there latex beads recommended for phagocytosis, and if so, what size is recommended?
1 answer-
There is no performance testing of latex beads for phagocytosis. Information found indicates that internally, there are no specific protocols or recommendations for a phagocytosis method using the listed latex beads. However, references to literature where the products have been used in research are provided. The maximum excitation and emission for one of the latex bead products, L3280, are also mentioned. Additionally, three products (catalog numbers 59769, 43302, and 89904) were found on the company website, with variations in bead size and fluorescence. For more details, you may refer to the provided links and Knowledge Article 000007596.
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What is the recommended storage temperature?
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This product may be stored at room temperature. For products with no specified storage temperature given on the label or product page, ambient storage may be assumed. Products that require controlled temperature storage, such as refrigeration or freezer conditions, storage temperatures are published on the product label, Certificate of Analysis, the Safety Data Sheet (SDS).
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