L7381

Lektin aus Arachis hypogaea (Erdnuss)

FITC conjugate, lyophilized powder

• Synonym(e): Erdnuss-Agglutinin, PNA
• NACRES: NA.32
• UNSPSC Code: 12352202
• eCl@ss: 32039090
• Form: lyophilized powder

Eigenschaften

• conjugate: FITC conjugate
• Quality Segment: 200
• form: lyophilized powder
• composition: Protein, ~10% Lowry
• extent of labeling: 4-8 mol FITC per mol protein
• storage temp.: −20°C

Beschreibung

General description

Lectins are carbohydrate-binding proteins, omnipresent, found in fungi, plants and animals. The structure of lectin is diversely studied in plants and animals. The secondary structure of this protein is rich in β-strands and possesses a carbohydrate binding sites on the surface.

Application

Lectin from Arachis hypogaea (peanut) has been used:

• to determine the acrosomal status (presence or absence of acrosomal matrix corresponding to intact or acrosome-reacted spermatozoa) on viable sperm cells
• to probe cryosections of mediastinal lymph nodes (mLNs) with fluorochrome-labeled PNA and anti-B220 to detect germinal centers (GCs)
• to visualize the cone outer segments of pre-treated retinal sections

Biochem/physiol Actions

Lectin is known to be useful in glycoconjugate characterizing, imaging and targeting. Its use in a microarray assay, enable efficient glycome profiling. This is because of its specific interaction with oligosaccharides, glycoproteins and glycolipids. In plants and fungi, lectin defends against pathogens/feeders. Lectin participates in host recognition and tissue adhesion, thereby aids in the pathogenesis of microorganism.

PNA verklumpt keine normalen humanen Erythrozyten, bewirkt aber eine starke Verklumpung (Agglutination) von mit Neuraminidase behandelten Erythrozyten. PNA weist eine potente Anti-T-Aktivität auf, ähnlich der Anti-T-Aktivität in Humanseren. Das Lektin kann für die Unterscheidung zwischen humanen Lymphozyten-Untergruppen verwendet werden.

Physical form

Contains phosphate buffer salts and NaCl

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• PSA (Persönliche Schutzausrüstung): Eyeshields, Gloves, type N95 (US)