LGC600524

LGC E.Cloni 10G Elite Electrocompetent Cells

pkg of 48 reactions (8 x 150 µL), Cfu/μg >2 x 10¹⁰

• Synonym(e): High Efficiency competent cells, DNA transformation, Electrocompetent E.Coli, Electroporation, competent cells
• UNSPSC Code: 41106202
• NACRES: NB.43
• Biological source: Escherichia coli
• Growth mode: growth or suspension
• Manufacturer/tradename: Lucigen 60052-4

Eigenschaften

• biological source: Escherichia coli
• manufacturer/tradename: Lucigen 60052-4
• packaging: pkg of 48 reactions (8 x 150 µL)
• growth mode: growth or suspension
• technique(s): microbiological culture: suitable
• cell transformation: competent cell type: electrocompetent; transformation efficiency: 2 x 10¹⁰ cfu/μg
• shipped in: dry ice
• storage temp.: -65 to -96°C

Beschreibung

General description

E. cloni electrocompetent cells incorporate a unique manufacturing technology that increases transformation efficiency, recombinant yields and reliability. These cells provide solutions for a wide range of applications at economical prices.

Application

E. cloni 10G Competent Cells. Library construction, cloning, subcloning, and plasmid isolation with or without blue/white screening.

Features and Benefits

• Optimized genetics for high yields: phage T1 resistant, endonuclease and recombination minus, blue/white screening-capable (without IPTG induction)
• Supports cloning methylated DNA
• Replace standard cloning strains (e.g., OP10, DH5α, DH10B, XL1-Blue, etc.)

Transformation efficiency: ≥ 2 x 10¹⁰ (cfu/µg pUC DNA)

Genotype: E. cloni 10G: F¯ mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 ɸ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697 galU galK rpsL (StrR) nupG λ- tonA

Components:


E. cloni 10G Elite Electrocompetent Cells


pUC 19 Transformation Control


Recovery Media

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• Was ist los?: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable