Direkt zum Inhalt
Merck

Wichtige Dokumente

Gesamtdokumentation anzeigen

Fortfahren mit

M0268

Minimum Essential Medium Eagle

With Earle′s salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Synonym(e):

MEM

Anmelden zur Ansicht der Organisations- und Vertragspreise.

Größe auswählen

Ansicht ändern
Größe/SKUVerfügbarkeitPreis
10 L
Warenkorb auf Verfügbarkeit prüfen
€ 41,70
10 x 1 L
Warenkorb auf Verfügbarkeit prüfen
€ 59,60
50 L
Warenkorb auf Verfügbarkeit prüfen
€ 185,00

Über diesen Artikel

NACRES:
NA.75
UNSPSC Code:
12352207

€ 41,70

Warenkorb auf Verfügbarkeit prüfen
​
Sonderanfertigung anfragen
Technischer Dienst
Benötigen Sie Hilfe? Unser Team von erfahrenen Wissenschaftlern ist für Sie da.
Unterstützung erhalten

form

powder

technique(s)

cell culture | mammalian: suitable

components

HEPES: no
phenol red: yes
Earle’s salts (5% CO2): yes
NaHCO3: no
L-glutamine: yes
sodium pyruvate: no

shipped in

ambient

storage temp.

2-8°C

General description

Listen to an Audio Overview
Minimum Essential Medium Eagle (MEM) is one of the most widely used synthetic cell culture media. MEM has been used for culturing several cells grown in monolayers. It is commonly used to grow attached cell lines, such as fibroblasts, in the presence of fetal bovine sera (FBS), calf, or horse sera.

Application

Minimum Essential Medium Eagle has been used:
  • in the isolation and culture of primary hepatocytes
  • to culture the human endometrial carcinoma cells Ishikawa (ATTC) and L-Wnt-3a cells (ATCC) that secrete Wnt family member 3a (Wnt-3a) ligand
  • to culture the AN3CA (endometrial cancer cell line) cells, and HEC1-B human (endometrial adenocarcinoma cell line) cells

Features and Benefits

  • Minimum Essential Medium Eagle (MEM) contains higher concentrations of amino acids to mimic the protein composition of cultured mammalian cells.
  • The formulation has been altered by optional elimination of calcium to enable the growth of cells in suspension culture.

Preparation Note

Formulated to contain 9.5 grams of powder per liter of medium.
Supplement with 2.2 g/L sodium bicarbonate.

Other Notes

Essentially the same as the original formula, except it is provided as a powder.

Ähnliche Artikel vergleichen

Vollständigen Vergleich anzeigen

Unterschiede anzeigen

1 of 1

Dieser Artikel
M0643M2645M4642
Minimum Essential Medium Eagle With Earle′s salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Sigma-Aldrich

M0268

Minimum Essential Medium Eagle

Minimum Essential Medium Eagle With Earle′s salts, L-glutamine, and non-essential amino acids, without sodium bicarbonate, powder, suitable for cell culture

Sigma-Aldrich

M0643

Minimum Essential Medium Eagle

Minimum Essential Medium Eagle HEPES Modification, with Earle′s salts, L-glutamine and 25 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture

SAFC

M2645

Minimum Essential Medium Eagle

Minimum Essential Medium Eagle With Hanks′ salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Sigma-Aldrich

M4642

Minimum Essential Medium Eagle

description

With Earle′s salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

description

With Earle′s salts, L-glutamine, and non-essential amino acids, without sodium bicarbonate, powder, suitable for cell culture

description

HEPES Modification, with Earle′s salts, L-glutamine and 25 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture

description

With Hanks′ salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

form

powder

form

powder

form

powder

form

powder

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

components

HEPES: no
phenol red: yes
Earle’s salts (5% CO2): yes
NaHCO3: no
L-glutamine: yes
sodium pyruvate: no

components

HEPES: no
Earle’s salts (5% CO2): yes
NaHCO3: no
phenol red: yes
L-glutamine: yes
sodium pyruvate: no

components

HEPES: 25 mM
phenol red: yes
L-glutamine: yes
NaHCO3: no
sodium pyruvate: no
Earle’s salts (5% CO2): yes

components

glucose: yes
Hanks’ salts (2% CO2): yes
L-glutamine: yes
Earle’s salts (5% CO2): no
NaHCO3: no
phenol red: yes
sodium pyruvate: no
stable glutamine: no
HEPES: no

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

classic media, classical media, FBS

classic media, classical media, FBS

classic media, classical media, FBS

Mammalian cell culture MEM 500mL, Minimum Essential Medium Eagle for lab, cell suspension culture media MEM, Minimum Essential Medium Eagle formulation with Hanks′ salts, MEM, classic media, classical media, FBS, Minimum Essential Medium Eagle with non-essential amino acids, SAFC EMEM for mammalian cell growth, high concentration amino acids MEM for cell culture, SAFC Minimum Essential Medium Eagle 500mL, Minimum Essential Medium Eagle 1L, EMEM cell culture media, MEM Eagle formulation for mammalian cells, 500mL MEM Eagle for scientific research, cell culture media MEM without calcium, EMEM for tissue culture applications

Still not finding the right product?

Explore all of our products under Minimum Essential Medium Eagle

Lagerklasse

13 - Non Combustible Solids

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Zulassungslistungen

Zulassungslistungen werden hauptsächlich für chemische Produkte erstellt. Für nicht-chemische Produkte können hier nur begrenzte Angaben gemacht werden. Kein Eintrag bedeutet, dass keine der Komponenten gelistet ist. Es liegt in der Verantwortung des Benutzers, die sichere und legale Verwendung des Produkts zu gewährleisten.

10035-04-8

CAS No.

    • Hier finden Sie alle aktuellen Versionen:

    Analysenzertifikate (COA)

    Lot/Batch Number
    0000493259
    25E417
    0000493235
    0000447923
    0000447924

    Die passende Version wird nicht angezeigt?

    Wenn Sie eine bestimmte Version benötigen, können Sie anhand der Lot- oder Chargennummer nach einem spezifischen Zertifikat suchen.

    Suche nach COA

    Besitzen Sie dieses Produkt bereits?

    In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

    Die Dokumentenbibliothek aufrufen

    Questions

    1–5 of 5 Questions  

    1. Does Product M0268, Minimum Essential Medium Eagle, have an expiration date?

      1 answer

      1. Yes, this product has an expiration date of 36 months from the date of manufacture. The expiration date can be found on the Certificate of Analysis.

        Helpful?

    2. What is the Department of Transportation shipping information for this product?

      1 answer

      1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

        Helpful?

    3. Does Product M0268, Minimum Essential Medium Eagle, contain phenol red?

      1 answer

      1. Yes, this product contains phenol red.  Phenol red is included in most medium as a visual pH indicator.  The medium should be changed when the color turns yellow (acidic) or purple (basic).

        Helpful?

    4. Does Product M0268, Minimum Essential Medium Eagle, contain sodium bicarbonate?

      1 answer

      1. No, this product does not contain sodium bicarbonate. It is recommended to add 2.2 g sodium bicarbonate per Liter of medium. Use either Product No. S5761 (powder) or S8761 (7.5% solution).

        Helpful?

    5. What is the formulation of Product M0268, Minimum Essential Medium Eagle?

      1 answer

      1. The formulation for this product can be found on the product datasheet.

        Helpful?

    Reviews

    No rating value

    Active Filters