M2634

Äpfelsäure-Dehydrogenase aus Schweineherz

buffered aqueous glycerol solution, ≥600 units/mg protein (biuret)

• Synonym(e): L-Malate: NAD⁺ oxidoreductase, MDH
• UNSPSC Code: 12352204
• NACRES: NA.54
• EG-Nummer: 1.1.1.37 (BRENDA, IUBMB)
• MDL number: MFCD00131567
• Specific activity: ≥600 units/mg protein (biuret)

Eigenschaften

• form: buffered aqueous glycerol solution
• Quality Segment: 200
• specific activity: ≥600 units/mg protein (biuret)
• cation traces: NH₄⁺: ≤10 μg/mg protein
• foreign activity: Glutamic-Oxalacetic Transaminase ≤0.01%, Glutamic-Pyruvic Transaminase ≤0.01%
• shipped in: wet ice
• storage temp.: 2-8°C

Beschreibung

General description

Malic dehydrogenase is a mitochondrial isozyme and an important catalyst in the tricarboxylic acid cycle.

Mitochondrial Isozyme

Application

Malic dehydrogenase has been used in a study to assess the comparative molluscicidal action of Ginko biloba extract on snail hosts. It has also been used in a study to investigate the effect of early feed restriction on metabolic programming and compensatory growth in broiler chickens.

Biochem/physiol Actions

Malic Dehydrogenase can be reversibly inactivated by treatment with pyridoxal 5′-phosphate at 25 °C.

Eukaryotic cells contain two different isozymes of malate dehydrogenase: mitochondrial (m-MDH) and soluble or cytoplasmic (s-MDH). This product consists of the mitochondrial form.
The enzyme catalyzes the following reaction:Oxaloacetate + β-NADH → L-Malate + β-NAD

Physical form

Solution in 50% glycerol containing 0.05 M potassium phosphate buffer, pH 7.5

Other Notes

One unit will convert 1.0 μmole of oxalacetate and β-NADH to _L-malate and β-NAD per min at pH 7.5 at 25 °C.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• Was ist los?: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• PSA (Persönliche Schutzausrüstung): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)