M6444

Anti-Mitofusin 2 antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(e): Anti-CMT2A, Anti-CMT2A2, Anti-CPRP1, Anti-KIAA0214, Anti-MARF, Anti-Mfn2
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD03455396
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: IP, WB CL
• Citations: 27

Eigenschaften

• biological source: rabbit
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~86 kDa
• species reactivity: human, mouse, rat
• concentration: ~1 mg/mL
• technique(s): immunoprecipitation (IP): 5-10 μg using HeLa human epitheloid carcinoma cell lysate, western blot (chemiluminescent): 0.5-1 μg/mL using extract of mouse and rat brain mitochondria
• UniProt accession no.: O95140
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... MFN2(9927) ; mouse ... Mfn2(170731) ; rat ... Mfn2(64476)

Beschreibung

General description

Mitofusin-2 (MFN2) is an outer mitochondrial membrane protein.

Anti-Mitofusin 2 is produced in rabbit using as immunogen a synthetic peptide corresponding to human mitofusin 2 with an N-terminal added cysteine, conjugated to KLH. Mitofusins (Mfn1 and Mfn2) form homotypic and heterotypic complexes for fusion in mitochondria.

Immunogen

synthetic peptide corresponding to amino acid residues 557-576 of human mitofusin 2 with an N-terminal added cysteine, conjugated to KLH. The corresponding sequence differs by one and two amino acids in rat and mouse mitofusin 2, respectively.

Application

Anti-Mitofusin 2 antibody has been used in immunoblotting. It may also be used in immunoprecipitation.

Anti-Mitofusin 2 antibody produced in rabbit has been used for Western blotting.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Biochem/physiol Actions

Mitofusin-2 (MFN2) plays an important role as a mitochondrial fusion protein and participates in many cell processes. It is mainly involved in regulating metabolic processes in the mitochondria. MFN2 prevents the formation of reactive oxygen species and controls endoplasmic reticulum stress. Mutations in the gene encoding this protein have been linked to diabetes and obesity.

MFN2 is essential for the maintenance of mitochondrial network and controls mitochondrial metabolism. Mfn2-dependent regulatory mechanism is disturbed in obesity by reduced Mfn2 expression. Mutations in MFN2 cause Charcot Marie-Tooth neuropathy type 2A, a neurological disorder that results from degeneration of axons in peripheral nerves.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable