This product is methylated Jurkat DNA (T cell leukemia). Unfortunately, we do not evaluate the molecular weight of the product. However, it's genomic DNA.
Fortfahren mit
M8570
Methylated Control DNA
50 ng/μL
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Über diesen Artikel
NACRES:
NA.55
UNSPSC Code:
23201100
Form:
liquid
Storage temp.:
−20°C
form
liquid
concentration
50 ng/μL
technique(s)
whole genome amplification: suitable
color
colorless
storage temp.
−20°C
General description
Fully methylated Jurkat DNA. 5-methyl-cytidine (5mC) content determined by mass spectrometry. See lot specific certificate of analysis or vial label for 5mC content of each lot.
Application
Methylated Control DNA has been used as a negative control in bisulfite sequencing of embryonic genomic DNA.
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Dieser Artikel | |||
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| description 50 ng/μL | description Useful as a control or standard for variety of epigenomic assays such as methylated or unmethylated DNA enrichment, bisulfite conversion or melt curve analysis to determine the status of global or locus- specific DNA methylation. | description The CpGenome Universal Methylated Mouse DNA Standard contains universally methylated mouse genomic DNA isolated from male Balb/c mice & enzymatically methylated at all CpGs by M.SssI methyltransferase. | description CpGenome 5-hmC DNA St&ard contains a linear, double-str&ed DNA (897 bp) with 100% hydroxymethylated cytosine. This st&ard can be used to calibrate applications aimed at analyzing & quantifying cytosine modifications. |
| form liquid | form - | form - | form - |
| concentration 50 ng/μL | concentration - | concentration - | concentration - |
| storage temp. −20°C | storage temp. - | storage temp. - | storage temp. - |
| color colorless | color - | color - | color - |
Lagerklasse
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Hello. Is this product a whole genomic DNA? Is it fragmented?
1 answer-
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What buffer is the Methylated Control DNA dissolved in?
1 answer-
This product is dissolved in a TE buffer consisting of 10 mM Tris-HCl with 1 mM EDTA.
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It has been written 50 ng/ul for concentration. What is the total volume (as ul or ml) and total probe amount (as ug or mg) in the vial. Could you inform me, please
1 answer-
The total volume is 16ul.
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What is the percentage of 5-Methylcytosine?
1 answer-
As mentioned on the product detail page, the % 5mC content is reported in the lot specific Certificate of Analysis, as well as the product label. The specification for 5mC is >/= 4.5%. The historical average is approximately 6.3%. Please see the sample Certificate of Analysis: https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/M8570-1VLSLCK7031.pdf
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